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alsD [Mon Aug 31 2015 13:57:09 GMT+0200 (CEST)]
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alsD [Mon Aug 31 2015 13:57:09 GMT+0200 (CEST)]

acetolactate decarboxylase
locus
BSU36000
pI
4.60
mw
28.00 kDa
function
overflow metabolism
product
acetolactate decarboxylase
essential
no
ec
4.1.1.5
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.5|Overflow metabolism]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|AlsR regulon], [SW|Rex regulon], [SW|stringent response]

    • The protein

    Catalyzed reaction/ biological activity

  • (2S)-2-hydroxy-2-methyl-3-oxobutanoate = (3R)-3-hydroxybutan-2-one + CO2 (according to Swiss-Prot)

    • Protein family

  • alpha-acetolactate decarboxylase family (according to Swiss-Prot)

    • Modification

  • phosphorylated on ser/ thr/ tyr [Pubmed|16493705], ''in vitro'' phosphorylated by [protein|search|PrkC] on Ser-88 [Pubmed|20389117]

    • Expression and Regulation

    Operon

  • ''[protein|search|alsS]-[protein|search|alsD]'' [Pubmed|7685336]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|7685336]

    • Regulation

  • induction by acetate ([protein|search|AlsR]) [Pubmed|7685336]
  • repressed as long as terminal electron acceptors are available for respiration ([protein|search|Rex]) [Pubmed|16428414]
  • subject to positive stringent control upon lysine starvation [Pubmed|20081037]
  • Note: since acetate formation requires ''[protein|search|ackA]'' activation by [protein|search|CcpA] there is an indirect effect of [protein|search|CcpA] on the ''alsSD'' operon: the operon is not expressed in ''[protein|search|ccpA]'' mutants

    • Regulatory mechanism

  • stringent response: due to presence of adenines at +1 and +2 positions of the transcript [Pubmed|20081037]
  • [protein|search|AlsR]: transcription activation in the presence of acetate [Pubmed|7685336]
  • [protein|search|Rex]: transcription repression if the ratio NADH2/NAD is high [Pubmed|16428414]

    • Biological materials

    Expression vector

  • for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP822, available in [SW|Stülke] lab

    • References

  • 19087206,10986270,16428414,16493705,7685336,16428414,20081037,20389117,24734205,22178965