SubtiBank SubtiBank
menA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

menA

probable 1,4-dihydroxy-2-naphthoate octaprenyltransferase
locus
BSU38490
pI
9.32
mw
33.68 kDa
protein length
311 aa Sequence Blast
gene length
933 bp Sequence Blast
function
biosynthesis of menaquinone
product
probable 1,4-dihydroxy-2-naphthoate octaprenyltransferase
essential
yes
ec
2.5.1.74
synonyms
ywaB, ipa-6d

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.8|Biosynthesis of menaquinone]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    3,950,726 → 3,951,661

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Protein family

  • menA family (according to Swiss-Prot)
  • Structure

  • [PDB|3BYV] (from ''Toxoplasma gondii'', 30% identity in a 25% fragment) [Pubmed|19197235]
  • [SW|Localization]

  • cell membrane (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|34DBBDD76A0B36867E5116FF17391DF70062F745|menA]
    description
    [Pubmed|9353933]
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    Biological materials

    Mutant

  • MGNA-B217 (ywaB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1216 NBRP B. subtilis, Japan]
  • References

  • 9353933,19197235