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ycbJ

similar to macrolide 2-phosphotransferase

locus: BSU_02520

pI: 4.57

mw: 34.33 kDa

protein length: 306 aa

gene length: 921 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.14|Resistance against toxins/ antibiotics/ based on similarity]

Gene

Coordinates: 275,838 276,758

The protein

Protein family

• aminoglycoside phosphotransferase family (with [protein|651F41AA16B0BB59A613D74302F441BF6D5BFF64|YtmP], according to UniProt)

Structure

• [PDB|5IGV] (from E. coli, 43% identity) [pubmed|28416110]

Expression and Regulation

Operons

• genes: [gene|3542BC20C11985B3CFAA4F930E8D794700ADFD2E|ycbC]-[gene|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|ycbD]-[gene|9C4932DA4DEF99263E57871A99684E3DAD93E1EC|ycbE]-[gene|372F2A420309DB0193AEF36A740EBA26410F97F2|ycbF]-[gene|1A65880F68898002EE8774F34EDC47F0243B7273|ycbG]-[gene|BA556E144975921DFDD598B0A7480C65176F0F14|ycbH]-[gene|349C99C258331962455100E2662DA2272E946C8A|ycbJ]
  description: [Pubmed|12044674]

  regulatory mechanism

  • [protein|1A65880F68898002EE8774F34EDC47F0243B7273|YcbG]: repression, [Pubmed|12044674], in [regulon|1A65880F68898002EE8774F34EDC47F0243B7273|YcbG regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|18840696], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • induced by D-glucarate/galactarate ([protein|search|YcbG]) [Pubmed|12044674]

Biological materials

Mutant

• MGNA-C032 (ycbJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2030 NBRP B. subtilis, Japan]
• BKE02520 ([gene|349C99C258331962455100E2662DA2272E946C8A|ycbJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCATTTCCCCTTTCTC, downstream forward: _UP4_TAAGAATTGACTTCGTTTCA
• BKK02520 ([gene|349C99C258331962455100E2662DA2272E946C8A|ycbJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCCATTTCCCCTTTCTC, downstream forward: _UP4_TAAGAATTGACTTCGTTTCA

References

• 12044674,18840696,28416110