ycbJ

similar to macrolide 2-phosphotransferase

Locus: BSU_02520

Isoelectric point: 4.57

Molecular weight: 34.33 kDa

Protein length: 306 aa

Gene length: 921 bp

Function: unknown

Product: unknown

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Coping with stress → Resistance against toxins/ antibiotics/ based on similarity

Gene

Coordinates: 275,838 276,758

The protein

Protein family

• aminoglycoside phosphotransferase family (with YtmP, according to UniProt)

Structure

• 5IGV (from E. coli, 43% identity) PubMed

Expression and Regulation

Operons

• Genes: ycbC-ycbD-ycbE-ycbF-ycbG-ycbH-ycbJ
  Description: PubMed

  Regulatory mechanism

  • YcbG: repression, PubMed, in YcbG regulon
  • AbrB: repression, PubMed, in AbrB regulon

  Regulation

  • induced by D-glucarate/galactarate (YcbG) PubMed

Biological materials

Mutant

• MGNA-C032 (ycbJ::erm), available at the NBRP B. subtilis, Japan
• BKE02520 (ycbJ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCCATTTCCCCTTTCTC, downstream forward: _UP4_TAAGAATTGACTTCGTTTCA
• BKK02520 (ycbJ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCCATTTCCCCTTTCTC, downstream forward: _UP4_TAAGAATTGACTTCGTTTCA

References

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