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ycbM
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ycbM

two-component sensor kinase
locus
BSU_02560
pI
9.75
mw
17.85 kDa
protein length
311 aa Sequence Blast
gene length
936 bp Sequence Blast
function
unknown
product
two-component sensor kinase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    279,059 279,994

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|60DA46B02BB5F71337AF5F34921E2E68EC062EC7|YcbL] (putative)
  • ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine

    • [SW|Domains]

  • [SW|Histidine kinase domain] (aa 92-310) (according to UniProt)

    • Modification

  • autophosphorylation on a His residue

    • Structure

  • [PDB|5C93] ([protein|E88978809272FAC2520DB4ABCA0554F8028F3451|WalK] from Lactobacillus plantarum, 27% identity) [pubmed|28994408]

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|60DA46B02BB5F71337AF5F34921E2E68EC062EC7|ycbL]-[gene|348F81B6ECB6FFBEC092A672FF5F40FE86918872|ycbM]-[gene|FE7AC936BE959D745FBB6A88EAD64408C7177125|ycbN]-[gene|621FA6E2A76BB74F9051FBDB6857ED56F65BAB15|ycbO]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-C035 (ycbM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2033 NBRP B. subtilis, Japan]
  • BKE02560 ([gene|348F81B6ECB6FFBEC092A672FF5F40FE86918872|ycbM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGCGGCCACCCATAGCACTG, downstream forward: _UP4_TAATCGTAAGAATTTCTTAA
  • BKK02560 ([gene|348F81B6ECB6FFBEC092A672FF5F40FE86918872|ycbM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02560 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AGCGGCCACCCATAGCACTG, downstream forward: _UP4_TAATCGTAAGAATTTCTTAA

    • References

  • 10094672,28994408