levB

endolevanase

locus: BSU_34460

pI: 4.99

mw: 58.81 kDa

protein length: 516 aa

gene length: 1551 bp

function: levan degradation

product: endolevanase

essential: no

ec: 3.2.1.64

synonyms: yveB

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,537,507 3,539,057

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i.e. 6-(beta-D-fructofuranosyl)-D-fructose, from the end of the chain (according to UniProt)

Protein family

• glycosyl hydrolase 32 family (with [protein|61D5B957E018D019AE0CE1D5F6C6A33EF982ED49|SacC] and [protein|8C2DD87E0351BBEE0FD8C0462087CCAD7459AA88|SacA], according to UniProt)

Structure

• [PDB|4FFF] (from Arthrobacter ureafaciens, 42% identity) [pubmed|22810228]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|AAA944BE7F01CE90F7730EECA16F3E4ED77D165A|sacB]-[gene|33BEC05F2129EBAF6699E847B0909A5A48F0E869|levB]
  description: [Pubmed|11739774]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2428811], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]: activation, [protein|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU]-P, [Pubmed|2428811], in [regulon|D343D2096664A972026D911E7A17A35C7B1CD1C9|DegU regulon]
  • [protein|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|SacY]: antitermination, /antitermination via binding to a [SW|RNA switch], in [regulon|EB330AB3DC6C8C3EC72C4CAFA2BC59CC485EE344|SacY regulon]

  regulation

  • the [SW|RNA switch] RNA is degraded by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [pubmed|29794222]

Biological materials

Mutant

• MGNA-B614 (yveB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1613 NBRP B. subtilis, Japan]
• BKE34460 ([gene|33BEC05F2129EBAF6699E847B0909A5A48F0E869|levB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGGGATTCACCTTTAT, downstream forward: _UP4_TAAAAACAGGGGCGGCGCAG
• BKK34460 ([gene|33BEC05F2129EBAF6699E847B0909A5A48F0E869|levB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGGGATTCACCTTTAT, downstream forward: _UP4_TAAAAACAGGGGCGGCGCAG

References

• 11739774,2428811,3039303,26773563,22810228