papA [2017-8-11 16:0:3]

Xaa-Pro amino-peptidase

locus: BSU24460

pI: 4.83

mw: 37.96 kDa

protein length: 353 aa

gene length: 1059 bp

function: degradation of proline-containing peptides

product: Xaa-Pro amino-peptidase

essential: no

ec: 3.4.11.9

synonyms: yqhT

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.3|Utilization of nitrogen sources other than amino acids] → [category|SW 2.3.3.4|Utilization of peptides]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.6|Coping with hyper-osmotic stress]

Gene

Coordinates: 2,538,697 → 2,539,758

Phenotypes of a mutant

• inactivation of ''[gene|33886A1EAC13B684E92BF94201C46617AD2844B3|papA]'' reduces sporulation efficiency to 1.2% that of wild type cells; delayed entry into sporulation [Pubmed|26735940]

The protein

Protein family

• peptidase M24B family (according to Swiss-Prot)

Paralogous protein(s)

• [protein|C95EEAC79B9E823BBBCF6B1960DABCCBB2AC03A4|PapB]

Structure

• [PDB|3Q6D] (the ''B. anthracis'' enzyme, 65% identity, 82% similarity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|449EEBF33F52B461F244331ACD140E68CF64EE7C|yqhR]-[gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]-[gene|33886A1EAC13B684E92BF94201C46617AD2844B3|papA]-[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]
  description: [pubmed|22383849]

• genes: [gene|D7503C73C0BC2E411C651E409E91C2E22E8842E5|yqhS]-[gene|33886A1EAC13B684E92BF94201C46617AD2844B3|papA]-[gene|3054CD45713BBD69C2A528DE24AF6AF37175FD9D|efp]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C447 (yqhT::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2445 NBRP B. subtilis, Japan]
• available in the [SW|Erhard Bremer] lab

Labs working on this gene/protein

• [SW|Erhard Bremer], Marburg University, Germany [http://www.uni-marburg.de/fb17/fachgebiete/mikrobio/molmibi Homepage]

References

• 23144141,26735940