SubtiBank SubtiBank
mecA

mecA

buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets ComK and ComS to ClpC-ClpP degradation machine (in log Phase), inhibits the transcriptional activity of Spo0A?P by direct interaction
locus
BSU11520
pI
4.21
mw
25.62 kDa
protein length
218 aa Sequence Blast
gene length
654 bp Sequence Blast
function
control of ComK degradation, regulation of competence
product
adaptor protein
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
  • Gene

    Coordinates
    1,229,068 → 1,229,724

    The protein

    Protein family

  • mecA family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|CE1C46944E242006383BA77B2C4E09045CB3B93F|YpbH]
  • [SW|Domains]

  • N-terminal domain (1 ... 120): recruitment of protein substrates [Pubmed|19801546]
  • C-terminal domain (121 ... 218): facilitates assembly of the [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP] oligomer and acts as a degradation tag [Pubmed|19801546]
  • Structure

  • [http://www.proteopedia.org/wiki/index.php/3jtp 3JTP] (C-terminal domain) [Pubmed|19801546]
  • [PDB|3PXG], [PDB|3J3U 3J3U] (the [protein|331993A875907C10C77105FD8DDD86D4412CE405|MecA]-[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC] complex) [Pubmed|23595989]
  • Expression and Regulation

    Operons

    genes
    [gene|331993A875907C10C77105FD8DDD86D4412CE405|mecA]
    description
    [Pubmed|8412687]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8412687], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Mutant

  • GP813 (spc), GP814 (aphA3) both available in the [SW|Stülke] lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 23375660,23479438,19609260
  • Original Publications

  • 11914365,9000055,17560370,19361434,9890793,2113920,8817496,8412687,11918817,10447896,12028382,8016067,10361283,11703662,9535081,19767395,19801546,8016066,19202088,8083167,21368759,23595989,16525504,12598648,21435029