mecA

buffering protein for development, dampens transitions to spore, biofilm exopolysaccharide and competence expression , targets [protein|search|ComK ]and [protein|search|ComS ]to the [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC]-[protein|search|ClpP ]degradation machine (in log phase), inhibits the transcriptional activity of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]-P by direct interaction

locus: BSU_11520

pI: 4.21

mw: 25.62 kDa

protein length: 218 aa

gene length: 657 bp

function: control of [protein|search|ComK ]degradation, regulation of competence

product: adaptor protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]

Gene

Coordinates: 1,229,068 1,229,724

The protein

Catalyzed reaction/ biological activity

• recruits protein for degradation by [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP] [pubmed|12598648]
• inhibits transcription activation of [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]-P (in complex with [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC]) [pubmed|29446505]

Protein family

• mecA family (with [protein|CE1C46944E242006383BA77B2C4E09045CB3B93F|YpbH], according to UniProt)

Paralogous protein(s)

• [protein|CE1C46944E242006383BA77B2C4E09045CB3B93F|YpbH]

[SW|Domains]

• N-terminal domain (1-120): recruitment of protein substrates [Pubmed|19801546]
• C-terminal domain (121-218): facilitates assembly of the [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC]-[protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP] oligomer and acts as a degradation tag [Pubmed|19801546]

Structure

• [PDB|3PXG] (the [protein|331993A875907C10C77105FD8DDD86D4412CE405|MecA]-[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC] complex) [pubmed|21368759]
• [PDB|3J3U] (the [protein|331993A875907C10C77105FD8DDD86D4412CE405|MecA]-[protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC] complex) [Pubmed|23595989]
• [PDB|3JTP](C-terminal domain) [Pubmed|19801546]

Expression and Regulation

Operons

• genes: [gene|331993A875907C10C77105FD8DDD86D4412CE405|mecA]
  description: [Pubmed|8412687]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8412687], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• GP813 (spc), GP814 (aphA3) both available in [SW|Jörg Stülke]'s lab
• BKE11520 ([gene|331993A875907C10C77105FD8DDD86D4412CE405|mecA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCCAACCTTCCCTTC, downstream forward: _UP4_TAGCAAACCGATTTCCTTCC
• BKK11520 ([gene|331993A875907C10C77105FD8DDD86D4412CE405|mecA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCCAACCTTCCCTTC, downstream forward: _UP4_TAGCAAACCGATTTCCTTCC

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 23375660,23479438,19609260

Original Publications

• 11914365,9000055,17560370,19361434,9890793,2113920,8817496,8412687,11918817,10447896,12028382,8016067,10361283,11703662,9535081,19767395,19801546,8016066,19202088,8083167,21368759,23595989,16525504,12598648,21435029,29165246,29446505