cell wall hydrolase (major autolysin) for cell elongation and separation, D,L-endopeptidase-type autolysin
function
major autolysin, cell elongation and separation
product
cell wall hydrolase (major autolysin),endopeptidase-type autolysin
Genomic Context
categories
[category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.2|Autolytic activity required for peptidoglycan synthesis (cell elongation)][category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape][category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis][category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.4|Endopeptidases][SW|Categories] containing this gene/protein
[SW|cell wall degradation/ turnover], [SW|cell wall synthesis]This gene is a member of the following [SW|regulons]
[SW|SigH regulon], [SW|SigI regulon], [SW|Spo0A regulon], [SW|WalR regulon]Gene
Coordinates on the chromosome (coding sequence)
1,018,998 -> 1,020,002
Phenotypes of a mutant
a ''[protein|search|cwlO] [protein|search|lytE]'' mutant is not viable [Pubmed|17581128,22139507]growth defect at high temperature [Pubmed|21541672]inactivation of ''[protein|search|lytE]'' strongly restores beta-lactam resistance in a ''[protein|search|sigM]'' mutant by delaying cell lysis [Pubmed|22211522]a ''[protein|search|lytE]'' mutation is synthetically lethal with ''[protein|search|ftsE]'' and ''[protein|search|ftsX]'' mutation (due to a lack of autolysin activity) [Pubmed|23869552,23855774]a ''[protein|search|lytE]'' mutation increases the cell separation defect of a ''[protein|search|lytF]'' mutant [Pubmed|23855774]cells are thinner (reduced diameter) as compared to the wild type [Pubmed|23869552]The protein
Catalyzed reaction/ biological activity
cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) [Pubmed|18266855]Protein family
nlpC/p60 family (according to Swiss-Prot)Paralogous protein(s)
the C-terminal D,L-endopeptidase domains of [protein|search|LytE], [protein|search|LytF], [protein|search|CwlS], and [protein|search|CwlO] exhibit strong sequence similarity[SW|Domains]
contains three N-acetylglucosamine-polymer-binding [SW|LysM domain]s [Pubmed|18430080]C-terminal D,L-endopeptidase domain [Pubmed|22139507]Effectors of protein activity
activity requires functional [protein|search|MreB] and [SW|MreBH] [Pubmed|23869552,16950129][SW|Localization]
binds the cell wall [Pubmed|21261835]localizes to cell septa, poles and lateral sidewall of the cell (via the N-terminal domain) [Pubmed|22139507]localization to lateral cell wall depends on the interaction with [SW|MreBH] [Pubmed|23869552][SW|Interactions]
[SW|MreBH]-[protein|search|LytE] along the cylindrical part of the cell wall [Pubmed|16950129][protein|search|FtsZ]-[protein|search|LytE] at the division septum [Pubmed|16950129][protein|search|PbpB]-[protein|search|LytE] at the division septum [Pubmed|16950129]Expression and Regulation
Operon
''lytE'' [Pubmed|9573210][SW|Sigma factor]
[protein|search|SigA] [Pubmed|9573210], [protein|search|SigH] [Pubmed|9573210], [protein|search|SigI] [Pubmed|21541672]Regulation
repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]induced at high temperature ([protein|search|SigI], [protein|search|WalR]) [Pubmed|24125693,21541672]Regulatory mechanism
[protein|search|WalR]: transcription activation [Pubmed|24125693,17581128][SW|Spo0A]: transcription repression [Pubmed|14651647]Biological materials
Mutant
1A790 ( ''lytE''::''cat''), [Pubmed|9457885], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A790&Search=1A790 BGSC]1A792 ( ''lytE''::''cat''), [Pubmed|1588906], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A792&Search=1A792 BGSC]1A1024 ( ''lytE''::''spec''), [Pubmed|20400548], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1024&Search=1A1024 BGSC]BKE09420 (''[protein|search|lytE]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab)FLAG-tag construct
GP2020 ''lytE-3xFLAG spec'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s labReferences
Reviews
18430080,23066944,18266855 Original publications
21261835,22211522,16950129,17581128,14594841,9457885,9573210,10322020,24125693,23199363,20059685,14651647,23869552,21541672,22139507,23855774 
