SubtiBank SubtiBank
darA [Tue Dec 13 2016 11:16:29 GMT+0100 (CET)]
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darA [Tue Dec 13 2016 11:16:29 GMT+0100 (CET)]

c-di-AMP-binding PII-like protein
locus
BSU00290
pI
6.00
mw
11.00 kDa
function
unknown
product
c-di-AMP-binding PII-like protein
synonyms
yaaQ

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.5|Targets of second messengers] → [category|SW 3.5.1|Targets of c-di-AMP]
  • [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]
  • [SW|Categories] containing this gene/protein

  • [SW|proteins of unknown function]
  • Gene

    Coordinates on the chromosome (coding sequence)
    39,871 -> 40,200

    The protein

    Effectors of protein activity

  • binds c-di-AMP [Pubmed|25433025]
  • Structure

  • [PDB|4RLE] (the ''B. subtilis'' protein in complex with c-di-AMP) [Pubmed|25433025]
  • [SW|Interactions]

  • forms homo-trimers [Pubmed|25433025]
  • Expression and Regulation

    Operon

  • ''[protein|search|darA]-[protein|search|yaaR]-[protein|search|holB]-[protein|search|yaaT]-[protein|search|yabA]-[protein|search|yabB]-[protein|search|yazA]-[protein|search|yabC]'' [Pubmed|22383849]
  • Biological materials

    Mutant

  • GP1712 (''[protein|search|darA]''::''cat''), available in [SW|Jörg Stülke]'s lab [Pubmed|25433025]
  • GP1713 (''[protein|search|darA]-[protein|search|yaaR]''::''cat''), available in [SW|Jörg Stülke]'s lab
  • GP2406 (''[protein|search|darA]''::''ermC''), available in [SW|Jörg Stülke]'s lab
  • Expression vector

  • pGP2601: IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844], available in [SW|Jörg Stülke]'s lab [Pubmed|25433025]
  • pGP1690: IPTG inducible expression, purification in ''E. coli'' with N-terminal cleavable His-tag, in [SW|pGP570], available in [SW|Jörg Stülke]'s lab
  • pGP2624: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
  • pGP2603: expression of ''darA''-Strep by [SW|pGP382] in ''B. subtilis'' suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • GP2010: expression of ''darA''-Strep in ''B. subtilis'' (chromosomal), suitable for [SW|SPINE], available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • Antibody

  • available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 25869574
  • Original publications

  • 22383849,25433025