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dnaK [Tue Dec 06 2016 16:21:54 GMT+0100 (CET)]
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dnaK [Tue Dec 06 2016 16:21:54 GMT+0100 (CET)]

class I heat-shock protein (molecular chaperone)
locus
BSU25470
pI
4.00
mw
65.00 kDa
function
protein quality control
product
class I heat-shock protein (molecular chaperone)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.2|Chaperones/ protein folding]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.5|Protein secretion]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

    • [SW|Categories] containing this gene/protein

  • [SW|chaperones/ protein folding], [SW|heat shock proteins], [SW|phosphoproteins], [SW|most abundant proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|HrcA regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,626,112 -> 2,627,947

    The protein

    Protein family

  • heat shock protein 70 family (according to Swiss-Prot)

    • Modification

  • phosphorylated on Tyr-601 by [SW|PtkA], dephosphorylated by [SW|PtpZ] [Pubmed|27148221,17726680]

    • Structure

  • [PDB|2V7Y] (from ''Geobacillus kaustophilus'', 87% identity) [Pubmed|18400763]
  • [PDB|4ANI] ([SW|DnaK]-[SW|GrpE] complex from ''Geobacillus kaustophilus'', 87% identity) [Pubmed|22544739]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • membrane-proximal (Spotty) [Pubmed|16479537]
  • recruited to the membrane after ethanol stress [Pubmed|22268681]

    • [SW|Interactions]

  • [protein|search|DnaK]-[protein|search|DnaJ] [Pubmed|27148221]
  • [SW|DnaK]-[SW|GrpE] [Pubmed|22544739]
  • [SW|DnaK]-[SW|PtkA] (for phosphorylation) [Pubmed|27148221]
  • [SW|DnaK]-[SW|PtpZ] (for dephosphorylation) [Pubmed|27148221]

    • Expression and Regulation

    Operon

  • ''[protein|search|hrcA]-[protein|search|grpE]-[protein|search|dnaK]-[protein|search|dnaJ]-[protein|search|yqeT]-[protein|search|yqeU]-[protein|search|yqeV]'' [Pubmed|9023197]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|1339421]

    • Regulation

  • induced by heat shock ([protein|search|HrcA]) [Pubmed|1339421]

    • Regulatory mechanism

  • [protein|search|HrcA]: transcription repression [Pubmed|1339421]

    • Additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Biological materials

    Mutant

  • BKE25470 (''dnaK''::''erm trpC2'') is available from the [http://bgsc.org Bacillus Genetic Stock Center]

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Wolfgang Schumann], Bayreuth University, Germany [http://www.genetik.uni-bayreuth.de/LSGenetik1/schumann_research.htm Homepage]

    • References

  • 1339421,12224648,10383760,9023197,7540247,7592421,16479537,1339421,17726680,22268681,15378759,22544739,18400763,27148221