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aag
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aag

hypoxanthine-DNA glycosylase, general stress protein, required for protection against paraquat stress
Locus
BSU_38620
Isoelectric point
6.12
Molecular weight
21.98 kDa
Protein length
196 aa Sequence Blast
Gene length
591 bp Sequence Blast
Function
DNA repair, survival of stress conditions
Product
hypoxanthine-DNA glycosylase
Essential
no
Synonyms
yxlJ

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,964,278 3,964,868

Phenotypes of a mutant

  • increased sensitivity of sporulating cells to the genotoxic effects of MMS PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • removes hypoxanthine, 3-alkylated purines, and 1,N6-ethenoadenine from DNA; hypoxanthine and 1,N6-ethenoadenine are the preferred substrates PubMed
  • protects sporulating cells from cytotoxic and genotoxic effects of MMS PubMed
  • Protein family

  • DNA glycosylase MPG family (single member, according to UniProt)
  • Structure

  • 3QI5 (the human enzyme in complex with DNA, 33% identity) PubMed
  • Localization

  • forespore PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon
  • SigG: sigma factor, PubMed, in SigG regulon
  • Regulation

  • expressed late during sporulation in the forespore SigG PubMed
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    Biological materials

    Mutant

  • MGNA-B756 (yxlJ::erm), available at the NBRP B. subtilis, Japan
  • BKE38620 (aag::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGAATCGACCTCTCCTTT, downstream forward: _UP4_TAGCATGTTTAAGGAAGAGG
  • BKK38620 (aag::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACGAATCGACCTCTCCTTT, downstream forward: _UP4_TAGCATGTTTAAGGAAGAGG
  • References

    Reviews

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    Original publications

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