SubtiBank SubtiBank
tsaD
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tsaD

required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with TsaC), universally conserved protein
locus
BSU05940
pI
5.02
mw
36.69 kDa
protein length
346 aa Sequence Blast
gene length
1038 bp Sequence Blast
function
tRNA modification
product
tRNA modification enzyme
essential
yes
ec
2.3.1.234
synonyms
ydiE,gcp

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]
  • Gene

    Coordinates
    643,258 → 644,298

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([protein|6CB350C335603D5F4434679F9D740BFECA738599|TsaB]-[protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|TsaD]-[protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|TsaE]) [Pubmed|23072323,21183954]
  • Modification

  • in vitro phosphorylated by [protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|TsaE] [pubmed|28890133]
  • Structure

  • [PDB|3ZET] (from ''Salmonella typhimurium'', 46% identity) [Pubmed|23471679]
  • Additional information

  • [SW|universally conserved protein]
  • Expression and Regulation

    Operons

    genes
    [gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]-[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]-[gene|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|ydiD]-[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]
    description
    [Pubmed|22383849]
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    References

    Publications on the corresponding protein from other species

  • 21183954,19376873,20824107,21619589,21285948,21625506,23056478,23471679,28890133,29741707