SubtiBank SubtiBank
tsaD
Save the date! 20th International Conference on Bacilli and Gram-Positive Bacteria. Washington, D.C. July 23-26, 2019.

tsaD

required for threonyl carbamoyl adenosine (t6A) modification of tRNAs that pair with ANN codons in mRNA (together with TsaC), universally conserved protein
locus
BSU_05940
pI
5.02
mw
36.69 kDa
protein length
346 aa Sequence Blast
gene length
1041 bp Sequence Blast
function
tRNA modification
product
tRNA modification enzyme
essential
yes
ec
2.3.1.234
synonyms
ydiE,gcp

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.5|Universally conserved proteins]
  • Gene

    Coordinates
    643,258 644,298

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • biosynthesis of the threonylcarbamoyladenosine (t6A) residue at position 37 of ANN-decoding tRNAs using L-threonylcarbamoyl-AMP ([protein|6CB350C335603D5F4434679F9D740BFECA738599|TsaB]-[protein|30393F16CFE89E7BAD233A0BD27B77D679F7E409|TsaD]-[protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|TsaE]) [Pubmed|23072323,21183954]
  • adenosine37 in tRNA + L-threonylcarbamoyladenylate --> AMP + H+ + N6-L-threonylcarbamoyladenosine37 in tRNA (according to UniProt)
  • Protein family

  • KAE1 / TsaD family (with [protein|6CB350C335603D5F4434679F9D740BFECA738599|TsaB], according to UniProt)
  • Modification

  • in vitro phosphorylated by [protein|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|TsaE] [pubmed|28890133]
  • Structure

  • [PDB|3ZET] (from ''Salmonella typhimurium'', 46% identity) [Pubmed|23471679]
  • [SW|Localization]

  • cytoplasm (according to UniProt)
  • Additional information

  • [SW|universally conserved protein]
  • Expression and Regulation

    Operons

    genes
    [gene|DE32F1DA1EA1164411E0605262381BD38A49656C|thiL]-[gene|E15BB518649B2B9CD44CEBBB4FA481E55BCE823C|tsaE]-[gene|6CB350C335603D5F4434679F9D740BFECA738599|tsaB]-[gene|C2283BDD806221A285A27F24F52F92F4D47F0C55|ydiD]-[gene|30393F16CFE89E7BAD233A0BD27B77D679F7E409|tsaD]
    description
    [Pubmed|22383849]
    view in new tab

    References

    Research papers

  • 31118925
  • Publications on the corresponding protein from other species

  • 21183954,19376873,20824107,21619589,21285948,21625506,23056478,23471679,28890133,29741707