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ripX [2018-02-11T16:22:13.000Z]
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ripX [2018-02-11T16:22:13.000Z]

site-specific integrase/recombinase, partitioning of the terminus region after replication
locus
BSU23510
pI
10.00
mw
33.84 kDa
protein length
296 aa Sequence Blast
gene length
888 bp Sequence Blast
function
chromosome partitioning
product
site-specific integrase/recombinase
essential
no
synonyms
ykqM

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.3|DNA condensation/ segregation]
  • Gene

    Coordinates
    2,448,591 → 2,449,481

    The protein

    Catalyzed reaction/ biological activity

  • resolution of chromosome dimers by site specific recombination at the dif site (located close to the terminus region on the chromosome)
  • Protein family

  • XerD subfamily (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|89746E312F20C81BE3CFDFCE7D99B01C375C198B|CodV]
  • Structure

  • [PDB|1A0P] (from ''E. coli'', 44% identity, complex with [protein|89746E312F20C81BE3CFDFCE7D99B01C375C198B|CodV]) [Pubmed|9311978]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • assembles on the chromosome at the dif site throughout the cell cycle [Pubmed|21239579]
  • Biological materials

    Mutant

  • MGNA-C414 (ripX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2412 NBRP B. subtilis, Japan]
  • 1A793 ( ''ripX''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A793&Search=1A793 BGSC]
  • 1A796 ( ''ripX''::''spec''), [Pubmed|11208805], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A796&Search=1A796 BGSC]
  • BKE23510 (Δ[gene|301568A5255A31538F52C46D7711EDAB2BD9CE90|ripX]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTTATTTGATCTTTCACTC, downstream forward: _UP4_TAGCCGCAGGTGGCTGCGGC
  • BKK23510 (Δ[gene|301568A5255A31538F52C46D7711EDAB2BD9CE90|ripX]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CTTTATTTGATCTTTCACTC, downstream forward: _UP4_TAGCCGCAGGTGGCTGCGGC
  • References

    Reviews

  • 23400100
  • Original publications

  • 10498718,16597952,11208805,21239579,11134515,9311978