SubtiBank SubtiBank
trmU
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trmU

tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase, involved in 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules
locus
BSU_27500
pI
4.73
mw
42.20 kDa
protein length
371 aa Sequence Blast
gene length
1116 bp Sequence Blast
function
tRNA modification
product
tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase
essential
yes
ec
2.8.1.-
synonyms
yrrA,mnmA

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,809,413 2,810,528

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with [protein|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|YrvO]) [Pubmed|25825430]
  • AH2 + ATP + S-sulfanyl-L-cysteinyl-[protein] + uridine34 in tRNA --> 2-thiouridine34 in tRNA + A + AMP + diphosphate + H+ + L-cysteinyl-[protein] (according to UniProt)
  • Protein family

  • MnmA/TRMU family (single member, according to UniProt)
  • Structure

  • [PDB|2HMA] (from ''Streptococcus pneumoniae'', 66% identity)
  • [SW|Localization]

  • cytoplasm(according to UniProt)
  • Expression and Regulation

    Operons

    genes
    [gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]-[gene|07DFF7D7BE1BACFB148F600400BDE5933BF1DD07|yrrB]-[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]
    description
    [Pubmed|22383849]
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    genes
    [gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]
    description
    [Pubmed|16885442]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16885442], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]
  • regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
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    References

  • 16885442,11948165,25825430