SubtiBank SubtiBank
trmU
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trmU

tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase, involved in 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules
locus
BSU27500
pI
4.73
mw
42.20 kDa
protein length
374 aa Sequence Blast
gene length
1122 bp Sequence Blast
function
tRNA modification
product
tRNA (5-methylaminomethyl-2-thiouridylate) methyltransferase
essential
yes
ec
2.8.1.-
synonyms
yrrA, mnmA

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    2,809,413 → 2,810,528

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • 2-thiouridine (s2U) modification of the wobble position in glutamate, glutamine and lysine tRNA molecules (together with [protein|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|YrvO]) [Pubmed|25825430]
  • Protein family

  • mnmA/TRMU family (according to Swiss-Prot)
  • Structure

  • [PDB|2HMA] (from ''Streptococcus pneumoniae'', 66% identity)
  • [SW|Localization]

  • cell membrane (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]-[gene|07DFF7D7BE1BACFB148F600400BDE5933BF1DD07|yrrB]-[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]
    description
    [Pubmed|22383849]
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    genes
    [gene|50930C56C27D22715620A350220E3C56ADB41020|cymR]-[gene|4F5945C3C8F18BD1B30D97CD516A06077C200B9D|yrvO]-[gene|2F5DFBE980FFF5810B76BCFCD189BA0F6C415B4F|trmU]
    description
    [Pubmed|16885442]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16885442], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]
  • regulation

  • [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
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    References

  • 16885442,11948165,25825430