acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)
function
acetoin utilization
product
acetoin dehydrogenase E3 component (dihydrolipoamide dehydrogenase)
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.2|Utilization of acetoin][SW|Categories] containing this gene/protein
[SW|utilization of specific carbon sources], [SW|sporulation proteins]This gene is a member of the following [SW|regulons]
[SW|AcoR regulon], [SW|CcpA regulon], [SW|SigL regulon]Gene
Coordinates on the chromosome (coding sequence)
882,266 -> 883,642
The protein
Catalyzed reaction/ biological activity
Protein N(6)-(dihydrolipoyl)lysine + NAD+ = protein N(6)-(lipoyl)lysine + NADH (according to Swiss-Prot)Protein family
class-I pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)Paralogous protein(s)
[protein|search|LpdV], [protein|search|PdhD][SW|Localization]
cytoplasm (according to Swiss-Prot)[SW|Interactions]
([protein|search|AcoA]-[protein|search|AcoB])-[protein|search|AcoC]-[protein|search|AcoL]Expression and Regulation
Operon
''[protein|search|acoA]-[protein|search|acoB]-[protein|search|acoC]-[protein|search|acoL]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed][SW|Sigma factor]
[protein|search|SigL] [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]Regulation
repressed by glucose ([protein|search|CcpA]) [Pubmed|10666464]induced by acetoin ([protein|search|AcoR]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed]induced under anaerobic conditions ([protein|search|Fnr]) [Pubmed|16428414]Regulatory mechanism
[protein|search|CcpA]: transcription repression [Pubmed|10666464][protein|search|AcoR]: transcription activation (interaction with [protein|search|SigL]-containing [SW|RNA polymerase]) [http://www.ncbi.nlm.nih.gov/sites/entrez/11274109 PubMed][protein|search|Fnr]: indirect positive regulation via [protein|search|NarG]-[protein|search|NarH]-[protein|search|NarJ]-[protein|search|NarI] [Pubmed|16428414]Additional information
the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]the mRNA is substantially stabilized upon depletion of [Rny|search|RNase Y] [PubMed|21815947]Labs working on this gene/protein
[SW|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]References
11274109,10368162,10666464,16428414,12884008,21815947 
