trigger enzyme: lipoic acid synthase and regulator of comE operon expression
function
lipid metabolism, required for the synthesis of branched-chain amino acids
product
trigger enzyme: lipoic acid synthase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.7|Biosynthesis and scavenging of lipoic acid][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.5|Trigger enzymes that control transcription in a yet unknown way][SW|Categories] containing this gene/protein
[SW|biosynthesis of cofactors], [SW|trigger enzyme], [SW|essential genes]Gene
Coordinates on the chromosome (coding sequence)
3,320,324 -> 3,321,220
Phenotypes of a mutant
reduced transformation efficiency [Pubmed|19028902], strong inhibition of growth on minimal media [Pubmed|19820084]The protein
Catalyzed reaction/ biological activity
Protein N(6)-(octanoyl)lysine 2 sulfur 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine 2 L-methionine 2 5'-deoxyadenosine (according to Swiss-Prot) Triacylglycerol H2O = diacylglycerol a carboxylate (according to Swiss-Prot) required for ''[SW|comEA]'' transcription [Pubmed|19028902]Protein family
Lipoyl synthase family (according to Swiss-Prot) AB hydrolase superfamily (according to Swiss-Prot)Structure
[PDB|4U0O] (from ''Thermosynechococcus elongatus'', 48% identity) [Pubmed|25100160][SW|Localization]
extracellular (signal peptide) [Pubmed|18957862]Biological materials
Mutant
MGNA-B578 (yutB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1577 NBRP B. subtilis, Japan]References
Reviews
Original publications
18957862,19028902,19820084,25100160 
