treA [Tue Dec 13 2016 11:16:31 GMT+0100 (CET)]

phospho-alpha-glucosidase

locus: BSU07810

pI: 4.90

mw: 65.00 kDa

function: trehalose utilization

product: phospho-alpha-glucosidase

essential: no

ec: 3.2.1.93

synonyms: treC

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.20|Utilization of trehalose]

[SW|Categories] containing this gene/protein

• [SW|utilization of specific carbon sources]

This gene is a member of the following [SW|regulons]

• [SW|CcpA regulon], [SW|PhoP regulon], [SW|TreR regulon]

Gene

Coordinates on the chromosome (coding sequence): 851,850 -> 853,535

The protein

Catalyzed reaction/ biological activity

• Alpha,alpha-trehalose 6-phosphate + H₂O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)

Protein family

• glycosyl hydrolase 13 family (according to Swiss-Prot)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operon

• ''[protein|search|treP]-[protein|search|treA]-[protein|search|treR]'' [Pubmed|8755887]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|8755887]

Regulation

• induced by trehalose ([protein|search|TreR]) [Pubmed|8755887]
• repressed by glucose ([protein|search|CcpA]) [Pubmed|18977770]
• expressed under conditions of phosphate limitation ([protein|search|PhoP]) [Pubmed|21636651]

Regulatory mechanism

• [protein|search|TreR]: transcription repression [Pubmed|8755887]
• [protein|search|CcpA]: transcription repression [Pubmed|18977770]
• [protein|search|PhoP]: transcription activation [Pubmed|21636651]

Biological materials

Mutant

• GP1118 (spc), available in the [SW|Stülke] lab

References

• 8755887,7651129,7751281,8917076,18977770