SubtiBank SubtiBank
pgsA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

pgsA

phosphatidylglycerophosphate synthase
locus
BSU16920
pI
5.16
mw
21.16 kDa
protein length
193 aa Sequence Blast
gene length
579 bp Sequence Blast
function
biosynthesis of phospholipids
product
phosphatidylglycerophosphate synthase
essential
yes
ec
2.7.8.5
synonyms
ymfN

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.2|Biosynthesis of phospholipids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • Gene

    Coordinates
    1,762,623 → 1,763,204

    Phenotypes of a mutant

  • some point mutations resulting in reduced PgsA activity lead to daptomycin resistance [Pubmed|21709092]essential [Pubmed|12682299]
  • depletion leads to hypersensitivity to sorbic acid stress [Pubmed|27818647]
  • The protein

    Catalyzed reaction/ biological activity

  • CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 3(3-sn-phosphatidyl)-sn-glycerol 1-phosphate (according to Swiss-Prot)
  • Protein family

  • CDP-alcohol phosphatidyltransferase class-I family (according to Swiss-Prot)
  • [SW|Localization]

  • cell membrane at the septum [Pubmed|19820159,15743965]
  • Biological materials

    Mutant

  • 1A815 ( ''pgsA''::''erm''), [Pubmed|12682299], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A815&Search=1A815 BGSC]
  • References

  • 19745567,16514141,19820159,15743965,21709092,27818647