cotSA

spore coat protein

locus: BSU_30910

pI: 7.81

mw: 42.75 kDa

protein length: 377 aa

gene length: 1134 bp

function: resistance of the spore

product: spore coat protein

essential: no

synonyms: ytxN,ytbA

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.8|Not yet assigned]

Gene

Coordinates: 3,160,761 3,161,894

The protein

Protein family

• [SW|glycosyltransferase 1 family] (according to UniProt)
• [SW|Glycosyltransferase 4 subfamily] (according to UniProt)

Paralogous protein(s)

• [protein|6911DFB27F6A23F3775DAC5C289EFA163FBD1E56|YtcC]

Expression and Regulation

Operons

• genes: [gene|2C17028E7DC755FFC190397AC6509E383C4DF99A|cotSA]-[gene|DC99E507696A1CB23140A1CBA45DA07D1F7C7D53|cotS]-[gene|5A13CEBB6820454E38DE81C1663078531B2AE4C2|ytxO]
  description: [Pubmed|9603889]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190,9603889], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|15383836], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

  regulation

  • expressed late during sporulation in the mother cell ([protein|search|SigK]) [Pubmed|15699190,9603889]

Biological materials

Mutant

• MGNA-A284 (ytfD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/284 NBRP B. subtilis, Japan]
• BKE30910 ([gene|2C17028E7DC755FFC190397AC6509E383C4DF99A|cotSA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACATAGCCTCCATTCCT, downstream forward: _UP4_AACAGATAGAAGGGGTGAAC
• BKK30910 ([gene|2C17028E7DC755FFC190397AC6509E383C4DF99A|cotSA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACATAGCCTCCATTCCT, downstream forward: _UP4_AACAGATAGAAGGGGTGAAC

References

• 11737650,10234840,15699190,9603889