SubtiBank SubtiBank
yloI
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yloI

Coenzyme A biosynthesis bifunctional protein CoaBC; putative phosphopantothenoylcysteine synthetase/decarboxylase
locus
BSU_15700
pI
6.01
mw
43.82 kDa
protein length
406 aa Sequence Blast
gene length
1221 bp Sequence Blast
function
biosynthesis of coenzyme A
product
putative phosphopantothenoylcysteine synthetase/decarboxylase
essential
yes
ec
4.1.1.36
synonyms
coaBC

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.4|Biosynthesis of coenzyme A]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    1,642,851 1,644,071

    Phenotypes of a mutant

  • essential [Pubmed|28189581]
  • The protein

    Catalyzed reaction/ biological activity

  • H+ + N-[(R)-4-phosphopantothenoyl]-L-cysteine --> CO2 + D-pantetheine 4'-phosphate (according to UniProt)
  • (R)-4'-phosphopantothenate + CTP + L-cysteine --> CMP + diphosphate + H+ + N-[(R)-4-phosphopantothenoyl]-L-cysteine (according to UniProt)
  • Protein family

  • N-terminal part: HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily (single member, according to UniProt)
  • C-terminal part: PPC synthetase family (single member, according to UniProt)
  • Structure

  • [PDB|1U7U] (from ''E. coli'', 41% identity in 53% fragment) [Pubmed|15530362]
  • Expression and Regulation

    Operons

    genes
    [gene|A57D2C1536F3E93CD687C28C59FF297355CAB303|yloC]-[gene|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|remA]-[gene|1BC994DE60A7BB35DEDD7154581A396D29AA94A7|gmk]-[gene|921933B6B893083F151AABDEDCBC65F2E55677BE|yloH]-[gene|2B67C63442F28F6BCBA3BA6DE4B563C73E8DF16C|yloI]-[gene|AF5ADDA2A98EC18BB72A81AA0336E639C0D1F430|priA]-[gene|3D41FB608DB70B9482312A1BBCCDBDA7FB713AFA|defA]-[gene|06971E9AAB033E2878914A76645F99E97CE8A90A|fmt]-[gene|1370D8783450D538C71E3DE8EA5B37DFEE073FF0|yloM]-[gene|5DEBD84E5937DB8B7F29C729FAA0076DF86A5451|yloN]

    description

  • [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B372 (yloI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1371 NBRP B. subtilis, Japan]
  • References

  • 22960854,15530362,28189581