SubtiBank SubtiBank
disA [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

disA [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

DNA integrity scanning protein, diadenylate cyclase, delays sporulation in the case of chromosome damage, the DisA-dependent checkpoint arrests DNA replication during B. subtilis spore outgrowth until the germinating spores genome is free of damage
locus
BSU00880
pI
5.00
mw
40.00 kDa
function
control of sporulation initiation
product
DNA integrity scanning protein, has diadenylate cyclase activity
essential
no
ec
2.7.7.85
synonyms
yacK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.3|Metabolism of signalling nucleotides]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.4|Heat shock proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|cell division], [SW|metabolism of signalling nucleotides], [SW|sporulation/ other], [SW|general stress proteins (controlled by SigB)], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|heat shock proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CtsR regulon], [SW|SigB regulon], [SW|SigM regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    107,476 -> 108,558

    The protein

    Catalyzed reaction/ biological activity

  • synthesis of c-di-AMP from two molecules of ATP [Pubmed|23192352,21566650]

    • Protein family

  • disA family (according to Swiss-Prot)

    • [SW|Domains]

  • contains a [SW|DAC domain] for the synthesis of c-di-AMP [Pubmed|18439896]

    • Effectors of protein activity

  • [protein|search|RadA] is an inhibitor of [protein|search|DisA] activity [Pubmed|23760274]

    • Structure

  • [PDB|3C23] [Pubmed|18439896]

    • [SW|Localization]

  • see a [http://download.cell.com/mmcs/journals/0092-8674/PIIS0092867406005034.mmc12.avi video] showing the movement of DisA in the cell (in real time) [Pubmed|16713562]
  • forms discrete globular foci in germinating spres that colocalize with the nucleoid [Pubmed|24244006]

    • [SW|Interactions]

  • forms octamers
  • [protein|search|RacA]-[protein|search|DisA] [Pubmed|12493822]
  • [protein|search|RadA]-[protein|search|DisA] [Pubmed|23760274], this interaction result in the inhibition of [protein|search|DisA] activity [Pubmed|23760274]

    • Expression and Regulation

    Operon

  • ''[protein|search|ctsR]-[protein|search|mcsA]-[protein|search|mcsB]-[protein|search|clpC]-[protein|search|radA]-[protein|search|disA]'' [Pubmed|8793870]
  • ''disA'' [Pubmed|17434969]

    • [SW|Sigma factor]

  • ''[protein|search|ctsR]'': [protein|search|SigA] [Pubmed|8793870], [protein|search|SigB] [http://www.ncbi.nlm.nih.gov/pubmed/8793870 PubMed1] [http://www.ncbi.nlm.nih.gov/pubmed/11544224 PubMed2]
  • ''disA'': [protein|search|SigM] [Pubmed|17434969]

    • Regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|11544224], induced by heat ([protein|search|CtsR]) [Pubmed|9987115]
  • expressed during [SW|sporulation] in the mother cell [Pubmed|24244006]
  • expressed during germination and spore outgrowth [Pubmed|24244006]

    • Regulatory mechanism

  • [protein|search|CtsR]: transcription repression [Pubmed|9987115]

    • Biological materials

    Mutant

  • GP2142 (''[protein|search|disA]''::''tet''), available in [SW|Jörg Stülke]'s lab
  • BKG2 (''[protein|search|radA]-[protein|search|disA]::spc''), available in [SW|Jörg Stülke]'s lab
  • 1A939 ( ''disA''::''tet''), [Pubmed|16713562], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A939&Search=1A939 BGSC]

    • Expression vector

  • IPTG inducible expression of His-''disA'' in ''E. coli'': pGP2563 (in [http://biochem.web.utah.edu/hill/links/pET19b.pdf pET19b]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

    • References

    Reviews

  • 22933559,18714086,25869574,23812326

    • Original publications

  • 24244006,8793870,9987115,16713562,23760274,11544224,17434969,18439896,16713555,12493822,21566650,23192352,23608499,25616256,26014055,27150552