mlpA

metallopeptidase, involved in regulation of protease gene expression

locus: BSU_16710

pI: 5.28

mw: 45.80 kDa

protein length: 409 aa

gene length: 1230 bp

function: control of proteolyticc activity

product: specific processing protease

essential: no

synonyms: ymxG

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]

Gene

Coordinates: 1,742,617 1,743,846

Phenotypes of a mutant

• fivefold increased levels of proteolytic activity in their growth medium [Pubmed|10666719]

The protein

Protein family

• [SW|Peptidase M16 family] (according to UniProt)

Structure

• [PDB|3HDI] (from B. halodurans, 64% identity) [pubmed|19913481]

Expression and Regulation

Operons

• genes: [gene|2AD0F2C0B0957A9E77EF9771D010ED77459B8CC0|mlpA]
  description: [Pubmed|8098035]

Biological materials

Mutant

• MGNA-A057 (ymxG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/57 NBRP B. subtilis, Japan]
• deletion mutant, available in [SW|van Dijl] lab
• BKE16710 ([gene|2AD0F2C0B0957A9E77EF9771D010ED77459B8CC0|mlpA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATTTGTTCCTCCTATTC, downstream forward: _UP4_TAAAAAGGAAAGCCTGCCCC
• BKK16710 ([gene|2AD0F2C0B0957A9E77EF9771D010ED77459B8CC0|mlpA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16710 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAATTTGTTCCTCCTATTC, downstream forward: _UP4_TAAAAAGGAAAGCCTGCCCC

References

• 10666719,10974125,8098035,19913481

Labs working on this gene/protein

• [SW|Jan Maarten van Dijl], Groningen, Netherlands