SubtiBank SubtiBank
nadF

nadF

NAD kinase
locus
BSU_11610
pI
5.99
mw
29.86 kDa
protein length
266 aa Sequence Blast
gene length
801 bp Sequence Blast
function
generation of NADP from NAD
product
inorganic polyphosphate/ATP-NAD kinase
essential
yes
ec
2.7.1.23
synonyms
yjbN,ppnK,ppnKA

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.11|Biosynthesis of NAD(P)]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    1,237,660 1,238,460

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP + NAD+ --> ADP + H+ + NADP+ (according to UniProt)
  • Protein family

  • NAD kinase family (with [protein|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|YtdI], according to UniProt)
  • Paralogous protein(s)

  • [protein|B729EFC0BF0D75765C2D2B0B4F397E0AEE11F1E7|YtdI]
  • Effectors of protein activity

  • allosterically activated by quinolinic acid
  • Structure

  • [PDB|2I1W] (from ''Listeria monocytogenes'', 60% identity) [Pubmed|17686780]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|E5A57660B69F9E9BC52FD9C8A5E3C8786CE96728|yjbL]-[gene|81BCF5C08EC1A580F5E05884BA830130FC7E46E9|sasB]-[gene|2A2640A0501ECEA44A3C0C4842FAC053FB422A1F|nadF]-[gene|2AECDA9C4D0E704976B9E2168722EC80FE7A256D|yjbO]
    description
    [Pubmed|18067544]

    regulation

  • expressed during exponential growth
  • view in new tab

    Biological materials

    Mutant

  • MGNA-B160 (yjbN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1159 NBRP B. subtilis, Japan]
  • 1A825 ( ''nadF''::''erm''), [Pubmed|12682299], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A825&Search=1A825 BGSC]
  • References

  • 12897004,18067544,17686780