SubtiBank SubtiBank
ybgE [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]
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ybgE [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

branched-chain amino acid aminotransferase
locus
BSU02390
pI
4.00
mw
39.00 kDa
function
biosynthesis of branched-chain amino acids
product
branched-chain amino acid aminotransferase
essential
no
ec
2.6.1.42
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]
  • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis/ acquisition of amino acids]
  • This gene is a member of the following [SW|regulons]

  • [SW|CodY regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    259,016 -> 260,086

    The protein

    Catalyzed reaction/ biological activity

  • l-leucine 2-oxoglutarate = 4-methyl-2-oxopentanoate L-glutamate (according to Swiss-Prot)
  • Protein family

  • class-IV pyridoxal-phosphate-dependent aminotransferase family (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|search|YwaA]
  • Structure

  • [PDB|3HT5] (from ''Mycobacterium tuberculosis'', 48% identity) [Pubmed|19923721]
  • Expression and Regulation

    Operon

  • ''ybgE'' [Pubmed|15060025]
  • Regulation

  • repressed by casamino acids [Pubmed|12107147]
  • repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]
  • Regulatory mechanism

  • [protein|search|CodY]: transcription repression [Pubmed|12618455,21699902,18083814]
  • Biological materials

    Mutant

  • a ''ybgE'' mutant and a ''[protein|search|bcd] [protein|search|ybgE] [protein|search|ywaA]'' triple mutant are available in [SW|Linc Sonenshein]'s lab
  • lacZ fusion

  • pGP248 (in [protein|search|pAC5]), available in [SW|Jörg Stülke]'s lab
  • References

  • 18083814,12618455,12670965,15060025,12107147,21699902,24163341,19923721