ybgE [2019-07-22 09:44:58]

branched-chain amino acid aminotransferase

locus: BSU_02390

pI: 4.95

mw: 39.52 kDa

protein length: 356 aa

gene length: 1071 bp

function: biosynthesis of branched-chain amino acids

product: branched-chain amino acid aminotransferase

essential: no

ec: 2.6.1.42

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.12|Biosynthesis/ acquisition of branched-chain amino acids]

Gene

Coordinates: 259,016 260,086

The protein

Catalyzed reaction/ biological activity

• l-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate (according to Swiss-Prot)

Protein family

• [SW|Class-IV pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

Paralogous protein(s)

• [protein|8163996FCB9D02F771E7A04A91D5720027260F12|YwaA]

[SW|Cofactors]

• PLP

Structure

• [PDB|3HT5] (from ''Mycobacterium tuberculosis'', 48% identity) [Pubmed|19923721]

Expression and Regulation

Operons

• genes: [gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ybgE]
  description: [Pubmed|15060025]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455,21699902,18083814], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]

  regulation

  • repressed by casamino acids [Pubmed|12107147]

Biological materials

Mutant

• MGNA-B939 (ybgE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1938 NBRP B. subtilis, Japan]
• a ''ybgE'' mutant and a ''[gene|A52E50104B18D0A8518218C52D9CC36FDDB29AAF|bcd] [gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ybgE] [gene|8163996FCB9D02F771E7A04A91D5720027260F12|ywaA]'' triple mutant are available in [SW|Linc Sonenshein]'s lab
• BKE02390 ([gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ybgE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTAATCAGCACACCTT, downstream forward: _UP4_TGAAAATCGAAAAAGAACCT
• BKK02390 ([gene|2A20EF701B9F78FC21A33D6BD8ED862323BA59A8|ybgE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATCTAATCAGCACACCTT, downstream forward: _UP4_TGAAAATCGAAAAAGAACCT

lacZ fusion

• pGP248 (in [SW|pAC5]), available in [SW|Jörg Stülke]'s lab

References

• 18083814,12618455,12670965,15060025,12107147,21699902,24163341,19923721