sdpR

transcriptional repressor ([SW|ArsR family]) of the [gene|29A3EC8FB0C763A973273AA14EAEB904847C3002|sdpR]-[gene|search|sdpI ]operon

locus: BSU_33790

pI: 7.33

mw: 10.10 kDa

protein length: 90 aa

gene length: 273 bp

function: regulation of protection against [protein|820635420B3980B620F3E3D7ACF7EDE9DC6275AD|SdpC]

product: transcriptional regulator ([SW|ArsR family])

essential: no

synonyms: yvbA

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins]

Gene

Coordinates: 3,467,054 3,467,326

The protein

Protein family

• [SW|ArsR family]

[SW|Domains]

• [SW|HTH arsR-type domain] (aa 1-87) (according to UniProt)

Effectors of protein activity

• [protein|3FB5839A7A80CF52E627FF1744E50490F5CC390F|SdpI] binds and sequesters [protein|29A3EC8FB0C763A973273AA14EAEB904847C3002|SdpR] if extracellular [protein|820635420B3980B620F3E3D7ACF7EDE9DC6275AD|SdpC] is present, this results in induction of the ''[gene|29A3EC8FB0C763A973273AA14EAEB904847C3002|sdpR]-[gene|3FB5839A7A80CF52E627FF1744E50490F5CC390F|sdpI]'' operon [Pubmed|16469701]

Structure

• [PDB|2CWE] (from Pyrococcus horikoshii, 34% identity)

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

additional information

• information on binding sites can be found in the [http://www.prodoric2.de/detail.php?acc=MX000211 PRODORIC2 database]

Expression and Regulation

Operons

• genes: [gene|29A3EC8FB0C763A973273AA14EAEB904847C3002|sdpR]-[gene|3FB5839A7A80CF52E627FF1744E50490F5CC390F|sdpI]
  description: [Pubmed|12817086]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16469701], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|16469701], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|29A3EC8FB0C763A973273AA14EAEB904847C3002|SdpR]: repression, [Pubmed|16469701], in [regulon|29A3EC8FB0C763A973273AA14EAEB904847C3002|SdpR regulon]

  regulation

  • induced by extracellular [protein|search|SdpC] ([protein|search|SdpR]) [Pubmed|16469701]

Biological materials

Mutant

• BKE33790 ([gene|29A3EC8FB0C763A973273AA14EAEB904847C3002|sdpR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGCGTTTTCTCCTTTAC, downstream forward: _UP4_TTATGAAGAAAAATATAATT
• BKK33790 ([gene|29A3EC8FB0C763A973273AA14EAEB904847C3002|sdpR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33790 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAGCGTTTTCTCCTTTAC, downstream forward: _UP4_TTATGAAGAAAAATATAATT

References

Reviews

• 20955377

Original Publications

• 12817086,16469701,10960106