SubtiBank SubtiBank
clpX [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]
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clpX [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

AAA unfoldase, ATP-dependent Clp protease, ATP-binding subunit (class III heat-shock protein)
locus
BSU28220
pI
4.00
mw
46.00 kDa
function
protein degradation
product
AAA unfoldase, ATP-dependent Clp protease, ATP-binding subunit
essential
no
synonyms

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
  • [SW|Categories] containing this gene/protein

  • [SW|proteolysis]
  • This gene is a member of the following [SW|regulons]

  • [SW|CtsR regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    2,884,781 -> 2,886,043

    Phenotypes of a mutant

  • increased thermotolerance due to increased stabiliy of [protein|search|Spx] and thus increased expression of ''[protein|search|trxA]'' [Pubmed|24417481]
  • the mutation suppresses the heat sensitivity of spores overexpressing ''[SW|cmpA]'' [Pubmed|26387458]
  • The protein

    Catalyzed reaction/ biological activity

  • ATPase/chaperone
  • Protein family

  • clpX chaperone family (according to Swiss-Prot) ClpX (IP004487) [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR004487 InterPro], AAA -type ATPase (IPR013093) [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR013093 InterPro] (PF07724) [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]
  • Paralogous protein(s)

  • [protein|search|ClpC], [protein|search|ClpE]
  • [SW|Domains]

  • AAA-ATPase [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]
  • Zinc finger [http://pfam.sanger.ac.uk/family?acc=PF06689 PFAM]
  • Structure

  • [PDB|1UM8] (from ''Helicobacter pylori'') [Pubmed|14514695]
  • [SW|Localization]

  • cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heat shock, colocalization with [protein|search|ClpP] [Pubmed|18786145,18689473]
  • [[File:ClpX.jpg‎ ]]
  • [SW|Interactions]

  • forms hexamers [Pubmed|26639779]
  • [protein|search|ClpP]-[protein|search|ClpX]
  • [protein|search|Spx]-[protein|search|ClpP]/[protein|search|ClpX] (degradation of [protein|search|Spx]) [Pubmed|17827297]
  • [SW|ClpX]-[SW|CmpA] (for [SW|ClpX]-[SW|ClpP]-dependent degradation of [SW|SpoIVA]) [Pubmed|26387458]
  • [SW|ClpX]-[SW|SpoIVA] (for [SW|ClpX]-[SW|ClpP]-dependent degradation of [SW|SpoIVA]) [Pubmed|26387458]
  • Expression and Regulation

    Operon

  • ''clpX'' [Pubmed|11325926]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|8973311]
  • Regulation

  • induced by heat stress ([protein|search|CtsR]) [Pubmed|9852015]
  • Regulatory mechanism

  • [protein|search|CtsR]: transcription repression [Pubmed|9852015]
  • Biological materials

    Mutant

  • MGNA-B019 (clpX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1018 NBRP B. subtilis, Japan]
  • GPUG2 (aphA3), available in [SW|Ulf Gerth]'s and [SW|Jörg Stülke]'s labs
  • ''clpX::kan'', ''clpX::spec'' and ''clpX::cat'' available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen]] Lab
  • GFP fusion

  • C-terminal GFP fusions (both single copy and 2th copy in ''amyE'' locus, also as CFP and YFP variants) available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen]] Lab
  • Labs working on this gene/protein

  • [SW|Leendert Hamoen], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen homepage]
  • References

    Reviews

  • 23375660,19680248,17302811,23479438,19609260,26639779
  • Original Publications

  • 12761164,10809708,9643546,11807061,14679237,18689476,16899079,8973311,19136590,11325926,8973311,9852015,18689473,20525796,15948963,18786145,24417481,24942655,25433860,25866879,14514695,26387458