SubtiBank SubtiBank
fusA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

fusA

elongation factor G, facilitates movement of tRNA–mRNA by one codon
locus
BSU01120
pI
4.61
mw
76.36 kDa
protein length
692 aa Sequence Blast
gene length
2076 bp Sequence Blast
function
translation
product
elongation factor G
essential
yes
synonyms
fus

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.11|Translation factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]
  • Gene

    Coordinates
    130,684 → 132,762

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • hydrolyses GTP
  • Protein family

  • EF-G/EF-2 subfamily (according to Swiss-Prot)
  • Modification

  • phosphorylation on Ser-213 AND Ser-302 AND Ser-569 AND Ser-680 AND (Thr-24 OR Thr-25) AND (Thr-43 OR Ser 48) [Pubmed|17218307], [Pubmed|17726680]
  • phosphorylated on Tyr-339 [Pubmed|26381121]
  • Structure

  • [PDB|1ELO] (from ''Thermus thermophilus'') [Pubmed|8070397]
  • [PDB|4BTC], [PDB|4BTD], the ''Thermus thermophilus'' EF-G–[SW|ribosome] complex in a pretranslocation state [Pubmed|23912278]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]
  • Expression and Regulation

    Operons

    genes
    [gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]-[gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL]-[gene|8049CACE85B2627A08CB4D8A8587D52E2DB24C7F|rpsG]-[gene|297844645CCCF40CB40E84339997F6610883DF4E|fusA]-[gene|E771685D6D41D48E0C0AF77F15B4F380820FCDC2|tufA]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]
  • regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • view in new tab

    Biological materials

    Expression vector

  • for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP840, available in [SW|Jörg Stülke]'s lab
  • for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP848, available in [SW|Jörg Stülke]'s lab
  • References

  • 24614227,17726680,17218307,20348921,8070397,23912278,15378759,25108354,26381121,27524615,26072700