SubtiBank SubtiBank
ywbG
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ywbG

holin-like auxilary protein
locus
BSU_38330
pI
9.56
mw
25.67 kDa
protein length
225 aa Sequence Blast
gene length
678 bp Sequence Blast
function
unknown
product
holin-like auxilary protein
essential
no
synonyms
ipa-22r
Outlinks
KEGGBsubCycSubtiListUniProt

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

    • Gene

    Coordinates
    3,933,577 3,934,254

    Phenotypes of a mutant

  • a ''[gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]'' mutant is delayed in pellicle formation [Pubmed|26060272]
  • a ''[gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG] [gene|E5E431AC5A7D03EEFF6D694CB25EED1570D739A2|yxaK]-[gene|5A43EDB738641C675D4136269F3638F9B609F238|yxaC] [gene|6F21B5E9EEF67E96808A1DEFA3A80DA2A0FF9462|pftA]-[gene|F6C3B267106E7510FABECF0B13ABF222901689A9|pftB]'' triple mutant is severely delayed in pellicle formation [Pubmed|26060272]
  • a ''[gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]'' overexpression strain has more robust pellicle formation [Pubmed|26060272]

    • The protein

    Protein family

  • YohK (E.coli)/YwbG (IPA-22R) (B.subtilis) family (with [protein|5A43EDB738641C675D4136269F3638F9B609F238|YxaC], according to UniProt)

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|7DA08C189F5524D4874E2F4828264AB2671A5460|ywbH]-[gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|YwbI]: activation, [Pubmed|26060272], in [regulon|CC55120C2AF455FA2AEAE39E21F57897B2B5BC39|YwbI regulon]

    • regulation

  • induced by acetate ([protein|search|YwbI]) [Pubmed|26060272]
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    Biological materials

    Mutant

  • MGNA-B224 (ywbG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1223 NBRP B. subtilis, Japan]
  • BKE38330 ([gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE38330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTTACAATTCCGATGAACA, downstream forward: _UP4_TAAAAAACTGTCCTCTCGAG
  • BKK38330 ([gene|2940E73F0BCAB69992BBD224402C95C2667757AD|ywbG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK38330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ACTTACAATTCCGATGAACA, downstream forward: _UP4_TAAAAAACTGTCCTCTCGAG

    • References

  • 26060272