sinI [Tue Feb 02 2016 13:51:57 GMT+0100 (CET)]

antagonist of SinR, drives SlrR from the SlrR(LOW) to the SlrR(HIGH) state

locus: BSU24600

pI: 6.00

mw: 6.00 kDa

function: control of biofilm formation

product: antagonist of SinR

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.6|Transition state regulators]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]

[SW|Categories] containing this gene/protein

• [SW|transcription factors and their control], [SW|transition state regulators], [SW|biofilm formation]

This gene is a member of the following [SW|regulons]

• [SW|AbrB regulon], [SW|ScoC regulon], [SW|Spo0A regulon]

Gene

Coordinates on the chromosome (coding sequence): 2,552,446 -> 2,552,619

Phenotypes of a mutant

• altered cell death pattern in colonies [Pubmed|23012477]

The protein

Paralogous protein(s)

• [protein|search|SlrA]

Structure

• [PDB|1B0N] (complex [protein|search|SinR]-[protein|search|SinI]) [Pubmed|9799632]

[SW|Interactions]

• [protein|search|SinR]-[protein|search|SinI] [Pubmed|9799632], K(D) 1.8 nM [Pubmed|23430750]

Expression and Regulation

Operon

• ''[protein|search|sinI]-[protein|search|sinR]'' [Pubmed|3125149]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|3125149]

Regulation

• activated under conditions that trigger sporulation by ([SW|Spo0A]) interaction [Pubmed|11751836],[Pubmed|21326214]
• repressed during exponential growth ([protein|search|ScoC]) [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467,11751836 PubMed]
• repressed during logrithmic growth ([protein|search|AbrB]) [http://www.ncbi.nlm.nih.gov/sites/entrez/7635837,11751836 PubMed]
• repressed during vegetative growth ([protein|search|SinR]) [Pubmed|1664536]

Regulatory mechanism

• [SW|Spo0A]: transcription activation, in a portion of cells [Pubmed|11751836]
• [protein|search|ScoC]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/1906467,11751836 PubMed]
• [protein|search|AbrB]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/7635837,11751836 PubMed]
• [protein|search|SinR]: transcription repression [Pubmed|1664536]

Additional information

• the mRNA is substantially stabilized upon depletion of [Rny|search|RNase Y] [PubMed|21815947]

Biological materials

Mutant

• GP959 (spc), available in [SW|Jörg Stülke]'s lab
• DS91 (spc) NCIB3610 derivate, available in [SW|Jörg Stülke]'s lab
• 1S98 ( ''sinI''::''kan''), [Pubmed|11466285], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S98&Search=1S98 BGSC]
• GP1663 (''[protein|search|yqhG]-[protein|search|sinI]-[protein|search|sinR]-[protein|search|tasA]''), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 20541494

Modelling of the [SW|SinI]]/[[SinR] switch

• 21095906

Original publications

• 23430750,23012477,21326214,21815947,3125149,15661000,18047568,11751836,1906467,11751836,7635837,11751836,10547280,15104138,9799632,20923420,8422983,24256735,26819068