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recG [2018-02-10T15:33:30.000Z]
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recG [2018-02-10T15:33:30.000Z]

ATP-dependent DNA helicase, branch migration translocase, required for DNA repair and chromosomal segregation
locus
BSU15870
pI
7.09
mw
77.96 kDa
protein length
682 aa Sequence Blast
gene length
2046 bp Sequence Blast
function
DNA repair and chromosomal segregation
product
ATP-dependent DNA helicase, branch migration translocase
essential
no
synonyms
ylpB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

    • Gene

    Coordinates
    1,659,810 → 1,661,858

    Phenotypes of a mutant

  • a [gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2] [gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG] double mutant is not viable [pubmed|28527403]

    • The protein

    Catalyzed reaction/ biological activity

  • binds and unwinds Holliday junction DNA [Pubmed|24770420]

    • Protein family

  • helicase C-terminal domain (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|AD1F39823ABCE2A222259B879513752942C59590|Mfd]

    • Structure

  • [PDB|1GM5] (from ''Thermotoga maritima'', 43% identity, 62% similarity)

    • Expression and Regulation

    Operons

    genes
    [gene|BC19D5B3764503298ED7136FC9F63A68F6AC464F|sdaAB]-[gene|8361D87307D2E8350945F592ABD24E2109E2E81A|sdaAA]-[gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • 1A897 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A897&Search=1A897 BGSC]
  • BKE15870 (Δ[gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCAATACCCTTAATGTTAG, downstream forward: _UP4_TGAGTATCAGAAGTTTTTGG
  • BKK15870 (Δ[gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCCAATACCCTTAATGTTAG, downstream forward: _UP4_TGAGTATCAGAAGTTTTTGG

    • References

  • 17853894,15533834,17640277,24770420,21170359,28527403