ymfI

Efp-5 aminopentanone reductase

Locus: BSU_16870

Isoelectric point: 5.22

Molecular weight: 25.43 kDa

Protein length: 242 aa

Gene length: 729 bp

Function: modification of Efp

Product: Efp-5 aminopentanone reductase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → Translation factor modification and maturation
• Information processing → Protein synthesis, modification and degradation → Protein modification → Protein modification/ other
• Lifestyles → Exponential and early post-exponential lifestyles → Swarming

Gene

Coordinates: 1,759,655 1,760,383

Phenotypes of a mutant

• defective in Swarming motility PubMed, this can be suppressed by mutations affecting earlier steps in Efp modification (gsaB, yaaO, yfkA, ynbB, ywlG) PubMed

The protein

Catalyzed reaction/ biological activity

• reduction of Efp-5 aminopentanone to Efp-5 aminopentanol PubMed

Protein family

• Short-chain dehydrogenases/reductases (SDR) family (according to UniProt)

Paralogous protein(s)

• YhxC, YcdF, YhdF
• FabG:
• YjdA:

Cofactors

• NADPH PubMed

Structure

• 2PNF (from Aquifex Aeolicus , 36% identity)

Biological materials

Mutant

• MGNA-B379 (ymfI::erm), available at the NBRP B. subtilis, Japan
• BKE16870 (ymfI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCTCTTTCCTTCTTTCC, downstream forward: _UP4_TGATCCGAAAATATTCGGTG
• BKK16870 (ymfI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCTCTTTCCTTCTTTCC, downstream forward: _UP4_TGATCCGAAAATATTCGGTG

References

Research papers

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