SubtiBank SubtiBank
prkC [Tue Dec 13 2016 11:16:33 GMT+0100 (CET)]
Save the date! 20th International Conference on Bacilli and Gram-Positive Bacteria. Washington, D.C. July 23-26, 2019.

prkC [Tue Dec 13 2016 11:16:33 GMT+0100 (CET)]

protein kinase C, induces germination of spores in response to DAP-type, and not to Lys-type cell wall muropeptides, stimulates WalR activity
71.00 kDa
germination in response to muropeptides
protein kinase

Genomic Context



  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW|Protein kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.4|Germination] → [category|SW|Additional germination proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]
  • [SW|Categories] containing this gene/protein

  • [SW|protein modification], [SW|germination], [SW|membrane proteins], [SW|phosphoproteins]
  • Gene

    Coordinates on the chromosome (coding sequence)
    1,651,142 -> 1,653,088

    Phenotypes of a mutant

  • unable to germinate in response to muropeptides [Pubmed|18984160]
  • reduced growth at high salt [Pubmed|25845974]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP a protein = ADP a phosphoprotein (according to Swiss-Prot)
  • phosphorylation of [protein|search|WalR] on Thr-101 to stimulate its activity [Pubmed|26102633]
  • Protein family

  • [SW|protein kinase] domain (according to Swiss-Prot)
  • [SW|Domains]

  • contains three C-terminal [SW|PASTA domain]s (aa 356-424, 425-492, 493-559) (binds muropeptides) [Pubmed|18984160]
  • Modification

  • phosphorylation on Thr-290 [Pubmed|17218307], autophosphorylation on multiple threonine residues [Pubmed|12842463,20389117]
  • Effectors of protein activity

  • activated by muropeptides [Pubmed|18984160]
  • [protein|search|GpsB], [SW|DivIVA], and [protein|search|EzrA] are required for stimulation of [protein|search|PrkC] activity [Pubmed|25845974]
  • Structure

  • [PDB|4EQM] (intracellular domain of the ''Staphylococcus aureus'' enzyme, 51% identity) [Pubmed|22701750]
  • [PDB|3PY9] (entire extra-cellular region of PrkC from ''Staphylococcus aureus'') [Pubmed|21208192]
  • [PDB|4X3F] (intracellular domain of the ''Mycobacterium tuberculosis'' enzyme, 36% identity, 68% similarity) [Pubmed|25586004]
  • [SW|Localization]

  • inner spore membrane [Pubmed|18984160]
  • membrane [Pubmed|12406230]
  • division septum [Pubmed|25845974]
  • [SW|Interactions]

  • [protein|search|AbrB]-[protein|search|PrkC] [Pubmed|24731262]
  • [protein|search|YvcK]-[protein|search|PrkC] [Pubmed|25012659]
  • [protein|search|GpsB]-[protein|search|PrkC] [Pubmed|25845974]
  • Expression and Regulation


  • ''[protein|search|prpC]-[protein|search|prkC]-[protein|search|cpgA]'' [Pubmed|16025310]
  • Biological materials


  • GP576 (spc), OMG302 (aphA3), available in [SW|Stülke] lab
  • 1A820 ( ''prkC''::''erm''), [Pubmed|12682299], available at [ BGSC]
  • 1A963 (no resistance), [Pubmed|12399479], available at [ BGSC]
  • 1A964 (no resistance), [Pubmed|12399479], available at [ BGSC]
  • Expression vector

  • for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP832, available in [SW|Stülke] lab
  • for expression/ purification of the kinase domain from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP849, available in [SW|Stülke] lab
  • for expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP1001, available in [SW|Stülke] lab
  • for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172]: pGP825, available in [SW|Stülke] lab
  • lacZ fusion

  • pGP829 (in [protein|search|pAC7]), available in [SW|Stülke] lab
  • References


  • 20972452,21372323,26429880,27148245
  • Phosphorylation of PrkC

  • 12842463,20563625
  • Targets of PrkC-dependent phosphorylation

  • 19246764,20070526,20389117,24390483,24731262,25012659,25278935,25845974,26102633
  • Phsiological role of PrkC

  • 12399479,12406230,19246764,12842463,18984160,26102633
  • Expression/ localization of PrkC

  • 16025310,24752279,26731423
  • Structure/ biochemistry of PrkC

  • 21208192,22111897,23793375,25668224,25586004,22701750