gcaD [Mon Aug 31 2015 13:51:48 GMT+0200 (CEST)]

bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

locus: BSU00500

pI: 5.65

mw: 49.00 kDa

function: cell wall metabolism

product: bifunctional N-acetylglucosamine-1-phosphate

essential: yes

ec: 2.7.7.23

synonyms: tms, tms26, glmU

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

syns: tms, tms26, glmU

[SW|Categories] containing this gene/protein

• [SW|cell wall synthesis], [SW|biosynthesis of cell wall components], [SW|essential genes]

This gene is a member of the following [SW|regulons]

• [SW|stringent response]

Gene

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate (according to Swiss-Prot)

Structure

• [PDB|1HM9] (from ''Streptococcus pneumoniae'', 49% identity) [Pubmed|11118459]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operon

• ''[protein|search|gcaD]-[protein|search|prs]-[protein|search|ctc]'' [Pubmed|8522540]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|8522540]

Regulation

• [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

Biological materials

Expression vector

• pGP2596: (IPTG inducible expression, purification in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab

References

• 1328164,8522540,11118459,21303765