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accA [Wed Nov 18 2015 10:12:27 GMT+0100 (CET)]
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accA [Wed Nov 18 2015 10:12:27 GMT+0100 (CET)]

acetyl-CoA carboxylase (alpha subunit)
locus
BSU29200
pI
6.00
mw
36.00 kDa
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (alpha subunit))
essential
yes
ec
6.4.1.2
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

    • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis of lipids], [SW|essential genes]

    • This gene is a member of the following [SW|regulons]

  • [SW|Spo0A regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,987,731 -> 2,988,708

    Phenotypes of a mutant

  • essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP acetyl-CoA HCO3- = ADP phosphate malonyl-CoA (according to Swiss-Prot)

    • Protein family

  • accA family (according to Swiss-Prot)

    • Structure

  • [PDB|2F9I] (from ''Staphylococcus aureus''; equivalent to [protein|search|AccD]-[protein|search|AccA], 64% identity) [Pubmed|16460018]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot), Membrane-proximal (Spotty) [Pubmed|16479537]

    • [SW|Interactions]

  • ([protein|search|AccA]-[protein|search|AccD])-[protein|search|AccB]-[protein|search|AccC] (acetyl-CoA carboxylase complex)

    • Expression and Regulation

    Operon

  • ''[protein|search|accD]-[protein|search|accA]'' [Pubmed|22383849]

    • Regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]

    • Regulatory mechanism

  • [SW|Spo0A]: transcription activation [Pubmed|14651647]

    • Biological materials

    Expression vector

  • purification from ''B. subtilis'' with an C-terminal Strep-tag, for [SW|SPINE], (in [SW|pGP382]): pGP1723, available in [SW|Jörg Stülke]'s lab

    • GFP fusion

  • GP1490 (spc, based on [SW|pBP43]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1487 (''spc'', based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • References

    Reviews

  • 15952903,17919287,12121720,15968460

    • Original Publications

  • 15066985,16460018,14651647,16479537,12663926,22383849