SubtiBank SubtiBank
accA
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

accA

acetyl-CoA carboxylase (alpha subunit)
locus
BSU29200
pI
6.09
mw
36.18 kDa
protein length
325 aa Sequence Blast
gene length
975 bp Sequence Blast
function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
product
acetyl-CoA carboxylase (alpha subunit))
essential
yes
ec
6.4.1.2
synonyms

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    2,987,731 → 2,988,708

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • ATP acetyl-CoA HCO3- = ADP phosphate malonyl-CoA (according to Swiss-Prot)
  • Protein family

  • accA family (according to Swiss-Prot)
  • Structure

  • [PDB|2F9I] (from ''Staphylococcus aureus''; equivalent to [protein|E4997526CC534E38413030E90F7A135ECF65DF40|AccD]-[protein|22B3039DF4D106F121DBC765F49878C9B324FDB2|AccA], 64% identity) [Pubmed|16460018]
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot), Membrane-proximal (Spotty) [Pubmed|16479537]
  • Expression and Regulation

    Operons

    genes
    [gene|E4997526CC534E38413030E90F7A135ECF65DF40|accD]-[gene|22B3039DF4D106F121DBC765F49878C9B324FDB2|accA]
    description
    [Pubmed|22383849]

    regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
  • view in new tab

    Biological materials

    Expression vector

  • pGP1021: purification from ''E. coli'' with an N-terminal His-tag, with TEV cleavage site, (in [SW|pETM-11]), available in [SW|Jörg Stülke]'s lab
  • pGP1024: purification from ''E. coli'' with an N-terminal Strep-tag, with TEV cleavage site, (in [SW|pGP172]), available in [SW|Jörg Stülke]'s lab
  • pGP1723: purification from ''B. subtilis'' with an C-terminal Strep-tag, for [SW|SPINE], (in [SW|pGP382]), available in [SW|Jörg Stülke]'s lab
  • GFP fusion

  • GP1490 (spc, based on [SW|pBP43]), available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP1487 (''spc'', based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • References

    Reviews

  • 15952903,17919287,12121720,15968460
  • Original Publications

  • 15066985,16460018,14651647,16479537,12663926,22383849,28579978