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epsD [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]
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epsD [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

extracellular polysaccharide synthesis
locus
BSU34340
pI
8.73
mw
43.00 kDa
function
biofilm formation
product
unknown
essential
no
synonyms
yveN

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.1|Matrix polysaccharide synthesis]
  • [SW|Categories] containing this gene/protein

  • [SW|biofilm formation]
  • This gene is a member of the following [SW|regulons]

  • [SW|AbrB regulon], [SW|EAR riboswitch], [SW|RemA regulon], [SW|SinR regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    3,525,250 -> 3,526,395

    Phenotypes of a mutant

  • smooth colonies on MsGG medium, no [SW|biofilm formation] [Pubmed|22113911]
  • The protein

    Protein family

  • glycosyltransferase 1 family (according to Swiss-Prot)
  • Expression and Regulation

    Operon

  • ''[protein|search|epsA]-[protein|search|epsB]-[protein|search|epsC]-[protein|search|epsD]-[protein|search|epsE]-[protein|search|epsF]-[protein|search|epsG]-[protein|search|epsH]-[protein|search|epsI]-[protein|search|epsJ]-[protein|search|epsK]-[protein|search|epsL]-[protein|search|epsM]-[protein|search|epsN]-[protein|search|epsO]'' [Pubmed|15661000]
  • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|15661000]
  • Regulation

  • repressed by [protein|search|SinR] [Pubmed|15661000]
  • Regulatory mechanism

  • [protein|search|SinR]: transcription anti-activation (prevents binding of [protein|search|RemA]) [Pubmed|23646920]
  • [protein|search|RemA]: transcription activation [Pubmed|23646920]
  • [protein|search|AbrB]: transcription repression [Pubmed|20817675]
  • Additional information

  • induction by sequestration of [protein|search|SinR] by [protein|search|SinI] or [protein|search|SlrA] [PubMed|15661000,19788541]
  • the [[EAR riboswitch]] (eps-associated [[RNA switch]]) located between' [protein|search|epsB]' and '[protein|search|epsC]' mediates processive antitermination and allows expression of the long eps operon [PubMed|20374491]
  • the '[protein|search|epsA]-[protein|search|epsB]-[protein|search|epsC]-[protein|search|epsD]-[protein|search|epsE]-[protein|search|epsF]-[protein|search|epsG]-[protein|search|epsH]-[protein|search|epsI]-[protein|search|epsJ]-[protein|search|epsK]-[protein|search|epsL]-[protein|search|epsM]-[protein|search|epsN]-[protein|search|epsO]' operon is not expressed in a '[protein|search|ymdB]' mutant [PubMed|21856853]
  • the amount of the mRNA is substantially decreased upon depletion of [Rny|search|RNase Y] (this is likely due to the increased stability of the '[protein|search|sinR]' mRNA) [PubMed|21815947]
  • Labs working on this gene/protein

  • [SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]
  • References

    Reviews

  • 20735481
  • =The EAR [SW|RNA switch]=

  • 20374491,20230605