spoIIGA

Pro-[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE] protease

locus: BSU_15310

pI: 8.55

mw: 34.70 kDa

protein length: 309 aa

gene length: 930 bp

function: maturation of [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]

product: Pro-[protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE] protease

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.3|Proteolysis during sporulation/ germination]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,603,779 1,604,708

The protein

Protein family

• peptidase U4 family (single member, according to UniProt)

Effectors of protein activity

• [protein|221829E084DA78ACE1C2DFCEB6E59EDA534FF55D|SpoIIGA] protease activity is stimulated by the interaction with [protein|9F338225E1286D530EAAAB504DEC8D5AFA06AC8D|SpoIIR] in the intermembrane space between the forespore and the mother cell [Pubmed|22111992]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|221829E084DA78ACE1C2DFCEB6E59EDA534FF55D|spoIIGA]-[gene|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|sigE]-[gene|5F24258282F3166B7696B9E9ABC1706E4D06C944|sigG]
  description: [Pubmed|1902213]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2512576], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|1902213], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]
  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

  regulatory mechanism

  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [PubMed|8288522,15687200], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]

  regulation

  • ''[SW|spoIIGA]'': expressed under conditions that trigger sporulation ([SW|Spo0A]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8288522,15687200 PubMed]

  additional information

  • the mRNA half-life is about 2.6 min [PubMed|24163345]

Biological materials

Mutant

• BKE15310 ([gene|221829E084DA78ACE1C2DFCEB6E59EDA534FF55D|spoIIGA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE15310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATCTGACTCCTTTCTTT, downstream forward: _UP4_TAATGTTCGCAAATGTCCGT
• BKK15310 ([gene|221829E084DA78ACE1C2DFCEB6E59EDA534FF55D|spoIIGA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK15310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATCTGACTCCTTTCTTT, downstream forward: _UP4_TAATGTTCGCAAATGTCCGT

References

Reviews

• 31350897

Original Publications

• 19581368,1537790,2448286,8288522,8231806,8002606,8730857,1744037,9287022,7585939,18296515,8288522,15687200