kamA

lysine 2,3-aminomutase

locus: BSU_19690

pI: 6.04

mw: 53.91 kDa

protein length: 471 aa

gene length: 1413 bp

function: unknown

product: lysine 2,3-aminomutase

essential: no

ec: 5.4.3.2

synonyms: yodO

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 2,139,454 → 2,140,869

The protein

Catalyzed reaction/ biological activity

• L-lysine --> (3S)-3,6-diaminohexanoate (according to UniProt)

Protein family

• [SW|Radical SAM superfamily] (according to UniProt)

[SW|Cofactors]

• Fe-S cluster [pubmed|29292548]
• PLP (according to UniProt)

Structure

• [PDB|2A5H] (from ''clostridium subterminale Sb4'' , 60% identity)

Expression and Regulation

Operons

• genes: [gene|13DC4296015A28975BC7697F134FB6ECD6B11489|yodT]-[gene|D8A050BF150C6E5B910C24C0E023F0829EC95BAD|yodS]-[gene|138832210AC94B17E081F3D0AF9BCFE3BE63E601|yodR]-[gene|161ADC907D4902001C8751F51CFA762828C26110|yodQ]-[gene|39749B5EADC3C056BAF232E0B586C65B989F7F77|yodP]-[gene|21589FEBDE54C66DE2A3DCFC2D7BF3AEEC84B44C|kamA]-[gene|A4A099EE3368891C2AD6C2D1DCBE7B238227417F|yokU]-[gene|0A33869E05CC9B49699555EA19D0905A55CA35B9|yozE]
  description: [Pubmed|22383849,14523133]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|14523133], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

  regulation

  • expressed early during sporuation in the mother cell ([protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]) [Pubmed|14523133]

Biological materials

Mutant

• MGNA-B443 (yodO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1442 NBRP B. subtilis, Japan]
• BKE19690 (Δ[gene|21589FEBDE54C66DE2A3DCFC2D7BF3AEEC84B44C|kamA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACGAACTCCTCCTCGCG, downstream forward: _UP4_ACTGAATGCGGAGGGGATTC
• BKK19690 (Δ[gene|21589FEBDE54C66DE2A3DCFC2D7BF3AEEC84B44C|kamA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19690 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAACGAACTCCTCCTCGCG, downstream forward: _UP4_ACTGAATGCGGAGGGGATTC

References

• 10839984,14523133