SubtiBank SubtiBank
kinB [2017-07-24 12:39:37]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

kinB [2017-07-24 12:39:37]

two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay, senses changes in respiratory activity
locus
BSU31450
pI
6.68
mw
47.71 kDa
protein length
429 aa Sequence Blast
gene length
1287 bp Sequence Blast
function
initiation of sporulation
product
two-component sensor kinase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.1|The kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.1|The kinases]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

    • Gene

    Coordinates
    3,230,067 → 3,231,353

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F]
  • mainly active in the older, inner regions of a colony (with [protein|B29CAD04FB89ACC482EFCD29D50EDDA19145CAA2|KinA]) [Pubmed|21097618]
  • sensing potassium level in the medium during initiation of [SW|sliding] [Pubmed|26152584]

    • [SW|Domains]

  • six transmembrane segments, C-terminal histidine phosphotransferase domain
  • selectivity filter sequence of potassium channels that is required for [SW|sliding] but not for [SW|sporulation] [Pubmed|26152584]

    • Modification

  • autophosphorylation on a His residue

    • Effectors of protein activity

  • activity is triggered at low respiratory activity, this depends on a functional interaction with the respiration apparatus [Pubmed|23599347]

    • Structure

  • [PDB|3D36] (from G. stearothermophilus, complex with [protein|CEFD10FAFA0DBC83CD2B61DAC9339FEE025B1611|Sda])

    • [SW|Localization]

  • cell membrane (integral membrane protein) [Pubmed|23599347]

    • Expression and Regulation

    Operons

    genes
    [gene|2111AC1AE49D1006E10DC127BF5B7A0327DE94A7|kinB]-[gene|18867F99B039D20DBCF004BC72E35CF0AE8FE467|kapB]
    description
    [Pubmed|8497199]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8497199], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12618455], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [regulon|stringent response|stringent response]: positive regulation, [Pubmed|23378509], in [regulon|stringent response|stringent response]
  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, [pubmed|29321771], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]

    • regulation

  • induced upon addition of decoyinine (positive [SW|stringent response]) [Pubmed|23378509]
    • view in new tab

    Biological materials

    Mutant

  • JH19980 (''kinB''::''tet'') [Pubmed|9334321]

    • References

  • 26152584,8576055,16166384,9299348,8497199,10094672,9426145,11069677,12618455,11902725,12618455,7592498,21097618,19101565,23378509,23599347,26152584