bacC

dihydroanticapsin 7-dehydrogenase

locus: BSU_37720

pI: 4.96

mw: 27.17 kDa

protein length: 255 aa

gene length: 768 bp

function: biosynthesis of the antibiotic bacilysin

product: dihydroanticapsin 7-dehydrogenase

essential: no

synonyms: ywfD,ipa-82d

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

Gene

Coordinates: 3,872,105 3,872,872

The protein

Catalyzed reaction/ biological activity

• oxidation of the C(7)-hydroxyl, penultimate step in bacilysin biosynthesis [Pubmed|23317005]
• L-dihydroanticapsin + NAD⁺ --> H⁺ + L-anticapsin + NADH (according to UniProt)

Protein family

• [SW|Short-chain dehydrogenases/reductases (SDR) family] (according to UniProt)

Paralogous protein(s)

• [protein|3161519994609DA9360ED6E073E4A4C8C6210EFB|YdaD], [protein|4DEFC2998464BF8327578C36A13A10DD277F991E|YkvO], [protein|6047F2493FCE3D2A9BDB1AD88E5926ECEED81036|YhxC], [protein|739B228743BC1FE9E6888E999BC0E4F615E36F9E|YhxD], [protein|B6FF689E65906186F3576B378650D713DB84EDDA|YcdF], [protein|EAEEA4DD9641919830A81185333A2610B964D37C|YhdF]
• [protein|0DD474462720CAEE2AE17017BD7CA385238EBC6F|YxbG]:
• [protein|0FD332269D2D6103E57ACE719E39977C68E38F0A|YvrD]:

[SW|Cofactors]

• NAD⁺ [pubmed|28158843]

Structure

• [PDB|5ITV] [pubmed|28158843]

Expression and Regulation

Operons

• genes: [gene|3F49F4AEFC49C2883EFA60FEB3E77591F184195B|bacA]-[gene|0EEE23875DB720D256D53F00D1AD5EF24B538B27|bacB]-[gene|20A7DBA142F3BF8DCAD9732BD908CE8CC79AA993|bacC]-[gene|2CE0C0484A70EEE40277996CD3D564A84FEAF5B3|bacD]-[gene|4D2AD84E74888EC61E44DE5B8E31812F43250B05|bacE]-[gene|99434727013FCCD6E570AE550560A8546F7F2A41|bacF]
  description: [Pubmed|12372825]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|19801406], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY]: repression, [Pubmed|12372825,21709425], in [regulon|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|CodY regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|12697329,21709425], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC]: repression, [Pubmed|19801406], in [regulon|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|ScoC regulon]

  regulation

  • expression of the operon is strongly induced at the beginning of [SW|biofilm formation] [pubmed|31113899]

Biological materials

Mutant

• MGNA-B669 (ywfD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1668 NBRP B. subtilis, Japan]
• BKE37720 ([gene|20A7DBA142F3BF8DCAD9732BD908CE8CC79AA993|bacC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTTTTATCGGTGAGGTTCA, downstream forward: _UP4_CAATAGAGAAGGAGTGTTTT
• BKK37720 ([gene|20A7DBA142F3BF8DCAD9732BD908CE8CC79AA993|bacC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTTTTATCGGTGAGGTTCA, downstream forward: _UP4_CAATAGAGAAGGAGTGTTTT

References

• 15609023,12372825,21948839,19801406,28158843