lipB

extracellular esterase, lipase

locus: BSU_08350

pI: 9.68

mw: 22.22 kDa

protein length: 210 aa

gene length: 633 bp

function: lipid degradation

product: extracellular esterase, lipase

essential: no

synonyms: yfiP

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.4|Utilization of lipids/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

Gene

Coordinates: 910,019 910,651

The protein

Catalyzed reaction/ biological activity

• triacylglycerol + H₂O --> diacylglycerol + fatty acid + H⁺ (according to UniProt)

Protein family

• [SW|AB hydrolase superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|0DB03F47C7AA45BF52653B1C0B2C5025960C53D7|Lip]

Structure

• [PDB|2QXT] ([protein|0DB03F47C7AA45BF52653B1C0B2C5025960C53D7|Lip], 75% identity) [pubmed|18053819]

Expression and Regulation

Operons

• genes: [gene|2053E27BE6837D2B167960C9B07C8B7B7BBABE25|lipB]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C304 (lipB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2302 NBRP B. subtilis, Japan]
• BKE08350 ([gene|2053E27BE6837D2B167960C9B07C8B7B7BBABE25|lipB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTATTCCCCCAAAAA, downstream forward: _UP4_TAATATCTTCAAAAAACAAC
• BKK08350 ([gene|2053E27BE6837D2B167960C9B07C8B7B7BBABE25|lipB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08350 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACGTTTATTCCCCCAAAAA, downstream forward: _UP4_TAATATCTTCAAAAAACAAC

References

• 12951259,11029590,11583117,10913700,18053819