ganR

transcriptional repressor of the galactan utilization operon

Locus: BSU_34170

Isoelectric point: 5.40

Molecular weight: 37.31 kDa

Protein length: 330 aa

Gene length: 993 bp

Function: regulation of galactan utilization

Product: transcriptional repressor (LacI family)

Essential: no

Synonyms: yvfJ,ganR

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of galactan
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other

Gene

Coordinates: 3,508,659 3,509,651

The protein

Protein family

• LacI family

Domains

• HTH lacI-type domain (aa 2-57) (according to UniProt)

Effectors of protein activity

• released from DNA upon binding of galactobiose PubMed

Structure

• 2HSG (B. megaterium CcpA, 25% identity) PubMed

Expression and Regulation

Operons

• Genes: ganR
  Description: PubMed

  Regulatory mechanism

  • GanR: repression, PubMed, in GanR regulon

  Regulation

  • induced by galactan (binding of galactobiose to GanR) PubMed

Biological materials

Mutant

• GP815 (ganR::spc) available in Jörg Stülke's lab
• BKE34170 (ganR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTATCTGCCTCCATTA, downstream forward: _UP4_TAAGGATGACTTAGGACACT
• BKK34170 (ganR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTATCTGCCTCCATTA, downstream forward: _UP4_TAAGGATGACTTAGGACACT

References

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