accC [Wed Sep 23 2015 18:43:08 GMT+0200 (CEST)]

acetyl-CoA carboxylase (biotin carboxylase subunit)

locus: BSU24340

pI: 4.00

mw: 49.00 kDa

function: production of malonyl-CoA, the substrate for fatty acid biosynthesis

product: acetyl-CoA carboxylase (biotin carboxylase subunit)

essential: yes

ec: 6.3.4.14

synonyms: yqhX

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[SW|Categories] containing this gene/protein

• [SW|biosynthesis of lipids], [SW|essential genes]

Gene

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• ATP biotin-carboxyl-carrier protein CO₂ = ADP phosphate carboxybiotin-carboxyl-carrier protein (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|YotE]

Structure

• [PDB|1BNC] (the protein from ''E. coli'') [Pubmed|7915138]

[SW|Interactions]

• ([protein|search|AccA]-[protein|search|AccD])-[protein|search|AccB]-[protein|search|AccC] (acetyl-CoA carboxylase complex)

Expression and Regulation

Operon

• ''[protein|search|accB]-[protein|search|accC]-[protein|search|yqhY]'' [Pubmed|7592499]

Biological materials

Mutant

• GP1478 (chromosomal ''accC''-Strep fusion, ''aphA''3), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

GFP fusion

• GP1491 (spc, based on [SW|pBP43]), available in [SW|Jörg Stülke]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

• GP1483 (''spc'', based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

References

Reviews

• 15952903,12121720,15968460

Original Publications

• 7592499,7915138