qcrB

menaquinol:cytochrome c oxidoreductase (cytochrome b subunit), component of the cytochrome bc complex

Locus: BSU_22550

Isoelectric point: 7.44

Molecular weight: 25.34 kDa

Protein length: 224 aa

Gene length: 675 bp

Function: respiration

Product: menaquinol:cytochrome c oxidoreductase (cytochrome b subunit)

Essential: no

Synonyms: bfcB,petB

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Respiration → Respiration/ other
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,363,913 2,364,587

The protein

Protein family

• cytochrome b family (with QcrC, according to UniProt)

Cofactors

• heme, covalently bound to Cys-43 PubMed

Structure

• 1VF5 (from Mastigocladus laminosus, 43% identity) PubMed

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: qcrA-qcrB-qcrC
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ResD: activation, PubMed, in ResD regulon
  • CcpA: repression, PubMed, in CcpA regulon
  • AbrB: repression, PubMed, in AbrB regulon

  Regulation

  • induced by oxygen limitation (ResD) PubMed

Biological materials

Mutant

• BKE22550 (qcrB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCAGTCATAAATTTTGTTCA, downstream forward: _UP4_TAAACTCTTGATCTGCGAAT
• BKK22550 (qcrB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCAGTCATAAATTTTGTTCA, downstream forward: _UP4_TAAACTCTTGATCTGCGAAT

References

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