single-strand DNA-binding proteinrequired to internalize and to recombine ssDNA with homologous resident duplex
function
required for efficient genetic transformation
product
single-strand DNA-binding protein
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue][SW|Categories] containing this gene/protein
[SW|DNA replication], [SW|genetic competence], [SW|DNA repair/ recombination], [SW|phosphoproteins]This gene is a member of the following [SW|regulons]
[SW|ComK regulon]Gene
Coordinates on the chromosome (coding sequence)
3,740,206 -> 3,740,547
The protein
Catalyzed reaction/ biological activity
binds and protects internalized foreign ssDNA [Pubmed|22373918]RecA-ATP in concert with [protein|search|DprA] and [protein|search|SsbA] catalyzes DNA strand exchange, with [protein|search|SsbB] as an accessory factor [Pubmed|25138221]Paralogous protein(s)
[protein|search|SsbA]Modification
phosphorylated on Arg-55 [Pubmed|22517742]phosphorylated on Tyr by [protein|search|PtkA], dephosphorylated by [protein|search|PtpZ] [Pubmed|16549871]Effectors of protein activity
phosphorylation strongly increases DNA-binding activity [Pubmed|16549871]Structure
[PDB|3VDY] (in complex with ssDNA) [Pubmed|22373918][SW|Localization]
localizes to cell poles in competent cells [Pubmed|16009133]localizes to the DNA entry pole during transformation [Pubmed|22373918]transiently localizes to the cell pole, and co-localizes with the DNA uptake machinery [Pubmed|17630974][SW|Interactions]
forms tetramers [Pubmed|22373918][protein|search|SsbB]-[SW|ComFA] [Pubmed|17630974][protein|search|SsbB]-[protein|search|CoiA] [Pubmed|17630974][protein|search|SsbB]-[protein|search|DprA] [Pubmed|17630974][protein|search|SsbB]-[protein|search|RecA] [Pubmed|17630974]Expression and Regulation
Operon
''[protein|search|ssbB]'' [Pubmed|22383849]''[protein|search|ssbB]-[protein|search|glcR]'' [Pubmed|14762004] (this is unlikely according to [Pubmed|22383849])Regulation
expressed at the onset of stationary phase [Pubmed|14762004]Regulatory mechanism
[protein|search|ComK]: transcription activation [Pubmed|11948146]Biological materials
Expression vector
for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth labReferences
Reviews
Original publications
16009133,17630974,22373918,16549871,14762004,11948146,22383849,22517742,23536821,23779106,25138221,26707201 
