SubtiBank SubtiBank
ysnA
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ysnA

ITPase
locus
BSU_28360
pI
4.95
mw
21.79 kDa
protein length
198 aa Sequence Blast
gene length
597 bp Sequence Blast
function
purine metabolism
product
ITPase
essential
no
ec
3.6.1.15
synonyms

Genomic Context

      

categories

  • [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]
  • Gene

    Coordinates
    2,900,545 2,901,141

    The protein

    Catalyzed reaction/ biological activity

  • H2O + XTP --> diphosphate + H+ + XMP (according to UniProt)
  • dITP + H2O --> dIMP + diphosphate + H+ (according to UniProt)
  • H2O + ITP --> diphosphate + H+ + IMP (according to UniProt)
  • Protein family

  • HAM1 NTPase family (single member, according to UniProt)
  • Structure

  • [PDB|2MJP] (from ''Methanococcus jannaschii'') [Pubmed|10404228], [PDB|4BNQ] (from E. Coli 52% identity)
  • Expression and Regulation

    Operons

    genes
    [gene|A18474CD50C2A1A83CF04C74098659BF1649B4DC|rph]-[gene|1BE734901DACBB56AB688C776F0AF8D298462518|ysnA]-[gene|13DF13F1EA21BC78035E5ED8B7AF2320235284A6|ysnB]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B030 (ysnA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1029 NBRP B. subtilis, Japan]
  • BKE28360 ([gene|1BE734901DACBB56AB688C776F0AF8D298462518|ysnA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCCTTTCTGTCTTTATT, downstream forward: _UP4_GAGGCATAAGGAGGAAGCTT
  • BKK28360 ([gene|1BE734901DACBB56AB688C776F0AF8D298462518|ysnA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCCTTTCTGTCTTTATT, downstream forward: _UP4_GAGGCATAAGGAGGAAGCTT
  • References

  • 10404228,11278832