SubtiBank SubtiBank
trxB
An ordered knock out library of all non-essential genes in B. subtilis is now available at Addgene. Information on ordering a copy can be found here. See Koo et al. 2017 for details about library construction.

trxB

thioredoxin reductase (NADPH)
locus
BSU34790
pI
4.99
mw
34.36 kDa
protein length
316 aa Sequence Blast
gene length
948 bp Sequence Blast
function
keeps TrxA|thioredoxin in the reduced state
product
thioredoxin reductase (NADPH)
essential
yes
ec
1.8.1.9
synonyms
yvcH

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.3|Electron transport/ other]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • Gene

    Coordinates
    3,573,207 → 3,574,157

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • Thioredoxin NADP = thioredoxin disulfide NADPH (according to Swiss-Prot)
  • Protein family

  • class-II pyridine nucleotide-disulfide oxidoreductase family (according to Swiss-Prot)
  • [SW|Cofactors]

  • FMN or FAD [Pubmed|21635694]
  • Structure

  • [PDB|4GCM] (from ''S. aureus, 71% identity'')
  • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    genes
    [gene|1BC439BBCD5FE19D5780519D7E4317C2EFF0D21B|trxB]
    description
    [pubmed|22383849]

    regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, [Pubmed|12642660], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]
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    References

    Reviews

  • 23899494
  • Original publications

  • 20084284,12642660,20525796,24401092