SubtiBank SubtiBank
ispF [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]
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ispF [Tue Dec 13 2016 11:16:30 GMT+0100 (CET)]

2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase, 5th step in the MEP pathway of isoprenoid biosynthesis
locus
BSU00910
pI
5.40
mw
16.00 kDa
function
MEP pathway of isoprenoid biosynthesis
product
2-C-methyl-D-erythrol-2,4-cyclodiphosphate synthase
essential
yes
ec
4.6.1.12
synonyms
yacN

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [SW|Categories] containing this gene/protein

  • [SW|biosynthesis of lipids], [SW|general stress proteins (controlled by SigB)], [SW|essential genes]
  • This gene is a member of the following [SW|regulons]

  • [SW|SigB regulon]
  • Gene

    Coordinates on the chromosome (coding sequence)
    110,480 -> 110,956

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP (according to Swiss-Prot)
  • Protein family

  • ispF family (according to Swiss-Prot)
  • Structure

  • [PDB|1T0A] (from ''Shewanella oneidensis'', 62% identity) [Pubmed|15502301]
  • Expression and Regulation

    Operon

  • ''[protein|search|yacL]-[protein|search|ispD]-[protein|search|ispF]'' [Pubmed|10482513]
  • [SW|Sigma factor]

  • [protein|search|SigB] [Pubmed|15805528]
  • Regulation

  • induced by stress ([protein|search|SigB]) [Pubmed|15805528]
  • References

  • 15805528,10482513,12270818,17458547,23840410,15502301