SubtiBank SubtiBank
queC
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queC

synthesis of the modified ribonucleotide queuosine
Locus
BSU_13720
Isoelectric point
5.15
Molecular weight
24.38 kDa
Protein length
219 aa Sequence Blast
Gene length
660 bp Sequence Blast
Function
tRNA modification
Product
unknown
Essential
no
Synonyms
ykvJ

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
1,439,448 1,440,107

The protein

Catalyzed reaction/ biological activity

  • 7-carboxy-7-deazaguanine + ATP + NH4+ --> 7-cyano-7-deazaguanine + ADP + H+ + H2O + phosphate (according to UniProt)
  • Protein family

  • queC family (single member, according to UniProt)
  • Structure

    Expression and Regulation

    Operons

    Description

    Regulatory mechanism

  • preQ1 riboswitch: antitermination, in the absence of queuosine PubMed, in preQ1 riboswitch
  • Regulation

  • repressed in the presence of queuosine (preQ1 riboswitch) PubMed
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    Biological materials

    Mutant

  • MGNA-A789 (ykvJ::erm), available at the NBRP B. subtilis, Japan
  • BKE13720 (queC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTCCTCTCCTTCTCC, downstream forward: _UP4_GAATATATGGTGATGAAAGG
  • BKK13720 (queC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATTCCTCTCCTTCTCC, downstream forward: _UP4_GAATATATGGTGATGAAAGG
  • References

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